Effect of tongyangxiao lotion on infected wound healing and expression of IL-1β and IκBα/p65 in rats
10.13431/j.cnki.immunol.j.20250057
- VernacularTitle:痛痒消洗剂对大鼠感染性创面愈合及IL-1β、IκBα/p65表达的影响
- Author:
Linyue WANG
1
;
Wenyue QI
;
Jihua GAO
;
Maosheng TIAN
;
Jiancheng XU
;
Yongkang AN
Author Information
1. 050013 石家庄,河北中医药大学第一附属医院肛肠科
- Publication Type:Journal Article
- Keywords:
Tongyangxiao lotion;
Anal fistula;
Wound healing;
Interleukin-1β;
IκBα;
p65;
Nuclear factor-κB
- From:
Immunological Journal
2025;41(6):387-394
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Tongyangxiao(TYX)lotion on the healing of infected wounds and the expression of interleukin(IL)-1β and inhibitor of nuclear factor κBα(IκBα)/p65 in rats.Methods Fifty rats were randomly assigned to the model group,potassium permanganate(PP)group,and low-,medium-,and high-dose(TYX-L,TYX-M,TYX-H)groups,with 10 rats in each group.An open infection model of full-thickness skin defects was established,and the rats in each group were treated with the corresponding medicinal solution for dressing changes once a day for a total of 14 days.The wound healing of rats was observed,and immunofluorescence,immunohistochemistry,and Western blotting were used to detect the nuclear translocation rate of p65 and the expression levels of IL-1β,p-IκBα/IκBα,and p-p65/p65 proteins in the granulation tissue.Results Compared with the model group,the wound healing rates of both the TYX-M group and the TYX-H group increased on the 7th day of treatment(P<0.05).Compared with the model group and the PP group,the wound healing rates of the TYX-L group,the TYX-M group and the TYX-H group increased on the 14th day of treatment(P<0.05).On the 7th day of treatment,the expression of IL-1β in the TYX-M group was lower than that in the PP group and the Model group(P<0.05),and the p-IκBα/IκBα ratio in the TYX-H group was lower than that in the PP group and the model group(P<0.05).The nuclear translocation rates of p65 in the TYX-L group,TYX-M group and TYX-H group were lower than those in the PP group and model group(P<0.05).On the 14th day of treatment,the p-p65/p65 ratio in the TYX-H group and the TYX-M group was lower than that in the model group(P<0.05),and the IL-1β in the TYX-L group,the TYX-M group,and the TYX-H group and p-IκBα/IκBα ratio in the TYX-H group were lower than those in the PP group and the model group(P<0.05).Conclusion The mechanism of TYX lotion in promoting the healing of infected wounds was associated with the suppression of the activation of NF-κB signaling pathway and alleviation of inflammatory responses.
