Impact of female reproductive tract microbiota and cytokine disorders on recurrent implantation failure
10.3760/cma.j.cn101441-20230810-00069
- VernacularTitle:女性生殖道菌群与细胞因子紊乱对反复着床失败的影响
- Author:
Fenting LIU
1
;
Shuo YANG
1
;
Ping ZHOU
1
;
Mingmei LIN
1
;
Rong LI
1
Author Information
1. 北京市生殖内分泌与辅助生殖技术重点实验室 辅助生殖教育部重点实验室(北京大学) 国家妇产科临床医学研究中心 北京大学第三医院妇产科生殖医学中心，北京 100191
- Publication Type:Journal Article
- Keywords:
Cytokines;
Repeated implantation failure;
Vaginal microbiota;
Uterine lavage microbiota;
Endometrial receptivity
- From:
Chinese Journal of Reproduction and Contraception
2024;44(1):8-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the changes in the functional composition of genital tract microbiota in patients with recurrent implantation failure (RIF) and its relationship with cytokines related to immune regulation.Methods:This was a case-control study. The study included patients from the Reproductive Medicine Center of Peking University Third Hospital, enrolled from August 2020 to January 2021. The enrolled patients were divided into control group ( n=20) and the RIF group ( n=15). The study examined the expression of flora and cytokines in vaginal and uterine lavage fluid by 16S rRNA sequencing and MSD electrochemiluminescence. Spearman analysis was used to observe the correlation between flora and differently expressional cytokines. Results:Compared with control group, there were statistically significant differences in the Shannon index of vaginal microbiota and uterine lavage fluid microbiota in the RIF group ( P=0.032, P=0.015). There was a statistically significant difference in β diversity of the bacterial community in uterine lavage fluid ( P=0.020). The expression level of monocyte chemotactic protein-4 (MCP-4) in the RIF group [(3.39±1.23) ng/L] was significantly higher than that in control group [(1.07±0.31) ng/L, P=0.044], while the expression level of interleukin (IL)-17A [(1.12±0.29) ng/L] was significantly lower than that in control group [(2.70±0.52) ng/L, P=0.040]. The expression levels of interferon (IFN)-γ [(4.70±2.10) ng/L], IL-10 [(0.20±0.10) ng/L], tumor necrosis factor-α [(0.84±0.13) ng/L], and IL-5 [(0.35±0.05) ng/L] in the RIF group in the uterine lavage were significantly higher than those in control group [(0.97±0.30) ng/L, P=0.049; (0.05±0.01) ng/L, P=0.009; (0.56±0.07) ng/L, P=0.045; (0.23±0.03) ng/L, P=0.029]. Correlation analysis revealed significant correlations between vaginal flora and MCP-4 ( r=-0.42, P=0.025), and in the microbial community of uterine cavity lavage fluid, there was a significant positive correlation between the Muribaculaceae genus and IL-5 ( r=0.51, P=0.017). Conversely, the Vulcaniibacterium genus, Cupriavidus genus, and Anoxybacillus genus exhibited a significantly negative correlation with IL-5 ( r=-0.55, P=0.010; r=-0.62, P=0.003; r=-0.45, P=0.041). Additionally, the genera Anoxybacillus, Geobacillus, Thermus, and Meiothermus showed a significantly negative correlation with IFN-γ ( r=-0.43, P=0.015; r=-0.38, P=0.035; r=-0.39, P=0.029; r=-0.38, P=0.035). Conclusion:The reproductive tract microbiota and corresponding cytokines in RIF patients are disrupted, and there is a correlation between the microbiota and cytokines.
