Study on the mechanism of allogeneic renal subcapsular transplantation of CD24+renal epithelial cells in the alleviation of ischemia-reperfusion-induced acute kidney injury in mice
10.13431/j.cnki.immunol.j.20250056
- VernacularTitle:同种异体肾被膜下移植CD24+肾上皮细胞缓解小鼠急性缺血再灌注肾损伤机制研究
- Author:
Yuxin ZHANG
1
;
Dawei LI
;
Mengting WANG
;
Shibo WANG
;
Wenming LIU
;
Hongqian MA
;
Qiuqiu ZHANG
;
Xiaoyan JIN
;
Hexin YAN
Author Information
1. 200000,上海交通大学医学院附属仁济医院麻醉科;200000 上海,麻醉医学教育部重点实验室(上海交通大学);201203,上海赛立维生物科技有限公司
- Publication Type:Journal Article
- Keywords:
Acute renal injury;
Cell therapy;
Macrophage;
CD24+;
Ischemia/reperfusion;
Creatinine;
Blood urea nitrogen;
Tumor necrosis factor-α;
Interleukin-6;
Mice
- From:
Immunological Journal
2025;41(6):377-386
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effect and potential mechanisms of allogeneic renal subcapsular transplantation of CD24+renal epithelial cells for the treatment of acute kidney injury(AKI)induced by ischemia-reperfusion(I/R).Methods CD24+renal epithelial cells were isolated from mouse kidneys using flow cytometric sorting and expanded by passaging.C57BL/6N mice were randomly divided into three groups:the normal control group(n=8,sham surgery only),the model control group(n=8,unilateral kidney I/R plus contralateral nephrectomy),and the CD24+cell treatment group(n=8,AKI model followed by renal subcapsular transplantation of CD24+cells).Mice were euthanized at 24 h after modeling and serum was collected to measure biochemical markers[serum creatinine(Scr),blood urea nitrogen(BUN),tumor necrosis factor-α(TNF-α),and interleukin-6(IL-6)].Renal tissues were subjected to pathological evaluation and macrophage staining.An M1-polarized macrophage model was established using mouse bone marrow-derived macrophages co-cultured with CD24+renal epithelial cells.The polarization state of macrophages was assessed by quantitative real-time polymerase chain reaction(qPCR)and flow cytometry.Results CD24+renal epithelial cells were successfully isolated and passaged stably.Compared with the normal control group,the model control group exhibited significantly elevated Scr and BUN levels and renal pathological damage.In contrast,the CD24+cell treatment group showed significant reduction in serum biochemical markers and pathological injury compared with the model control group,along with reduction in M1 macrophage infiltration in the kidneys(P<0.05,P<0.01).In vitro co-culture experiments demonstrated that in the CD24+co-culture group,the expression of M1 polarization-related markers in macrophages was significantly lower than that in the non-co-culture group,and the proportion of CD80+M1 macrophages in the co-culture group decreased(P<0.05,P<0.01).Conclusion Allogeneic renal subcapsular transplantation of CD24+renal epithelial cells can alleviate I/R-induced AKI by inhibiting M1 macrophage polarization through paracrine mechanisms.
