Determination of Six Volatile Compounds in Wuli Huichun Pills by Gas Chromatogra-phy-Mass Spectrometry Method
10.3870/j.issn.1004-0781.2025.09.008
- VernacularTitle:气相色谱-质谱法测定五粒回春丸中6种挥发性成分含量
- Author:
Shan LI
1
;
Haiyan LI
1
;
Yanan LIU
1
;
Haibo WANG
1
;
Qingguo RU
1
;
Xia HUANG
1
;
Ludi JIANG
1
Author Information
1. 河南省药品医疗器械检验院,国家药品监督管理局中药材及饮片质量控制重点实验室,河南省中药质量控制工程研究中心,郑州 450018
- Publication Type:Journal Article
- Keywords:
Wuli Huichun pills;
Volatile components;
Gas chromatography-mass spectrometry method
- From:
Herald of Medicine
2025;44(9):1418-1422
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a gas chromatography-mass spectrometry method for simultaneous determination of the contents of volatile components muscone,menthol,borneol,isoborneol,camphor,and carvone in Wuli Huichun pills,and to evaluate the quality of Wuli Huichun pills based on authenticity,effectiveness and safety.Methods The ethyl acetate extract was analyzed by HP-5MS capillary column(30 m×0.25 mm,0.25 μm)with temperature programming.The injection port temperature was set at 230℃,and the split ratio was set at 10∶1.The carrier gas was high-purity helium gas.The injection port was in constant flow mode,with a carrier gas flow rate of 1.0 mL·min-1.The detector was a mass spectrometer with a triple quadrupole in series.The ion source was an electron bombardment source(EI)with a temperature of 250℃.The collision gas was argon.The temperature of the mass spectrometry transmission interface was 250℃.Scanning mode was SIM mode,with a solvent delay of 2 minutes.Results The linear relationships of the six components were good(r>0.999 0),the average recoveries were 93.57%-97.96%,and the RSD was 0.73%-2.5%.Conclusion The method is simple,accurate,and efficient,and can be used for the quality evaluation of Wuli Huichun pills.
