Effects of blastocysts trophoectoderm biopsy on serum β-hCG levels of early pregnancy and perinatal outcomes in frozen embryo transplantation: a propensity score matching research
10.3760/cma.j.cn101441-20221119-00517
- VernacularTitle:滋养外胚层细胞活检对冻胚移植后妊娠早期血清β-hCG水平及围产期结局的影响：一项倾向评分匹配研究
- Author:
Ruxue YANG
1
;
Jiangdi HUANG
;
Yang LIU
;
Bingnan REN
;
Yichun GUAN
;
Lijun SUN
Author Information
1. 郑州大学第三附属医院生殖中心，郑州 450052
- Publication Type:Journal Article
- Keywords:
β-human chorionic gonadotropin;
Preimplantation genetic testing;
Trophoectoderm biopsy;
Frozen-thawed embryo transfer;
Perinatal outcome
- From:
Chinese Journal of Reproduction and Contraception
2023;43(12):1222-1228
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of trophoectoderm biopsy of blastocysts on serum β-human chorionic gonadotropin (β-hCG) in early pregnancy and perinatal outcome after frozen-thawed embryo transfer (FET) in the preimplantation genetic testing (PGT).Methods:It was a retrospective cohort study. Patients who underwent FET in the Reproductive Center of the Third Affiliated Hospital of Zhengzhou University from January 2017 to December 2021 were recruited. The patients were divided into two groups according to the progestation method: 308 patients underwent trophoectoderm biopsy (PGT group) and 802 patients underwent intracytoplasmic sperm injection (ICSI group). The patients were matched at 1∶2 with propensity score matching (PSM), then there were 300 patients in PGT group and 571 patients in ICSI group. The general conditions and perinatal outcomes were compared among PGT group and ICSI group before and after PSM. Multivariate linear regression was applied to analyze the effect of trophoectoderm biopsy to serum β-hCG level by day 14 after embryo transfer.Results:Before PSM, the female age in PGT group was significantly higher than that in ICSI group [(31.2±4.1) years vs. (30.4±4.3) years, P=0.007]. The anti-Müllerian hormone levels [(4.38±3.62) μg/L vs. (4.87±3.78) μg/L, P=0.049] and endometrial thickness on the day of embryo transfer [(9.1±1.6) mm vs. (9.6±1.6) mm, P<0.001] were lower in PGT group than in ICSI group. There was no significant difference in the general data between the two groups after PSM (all P>0.05). Before and after PSM, there was no significant difference in serum β-hCG level on day 14 after embryo transfer between PGT and ICSI groups of the all patients, clinical pregnancy patients, early abortion patients and live birth patients (all P>0.05). There were no significant differences in biochemical pregnancy rate, clinical pregnancy rate, early abortion rate and live birth rate between the two groups before and after matching (all P>0.05). Multivariate linear regression analysis showed that whether or not PGT was performed had no effect on serum β-hCG levels ( P=0.494), and female body mass index and type of blastocyst transferred had an effect on β-hCG levels (all P<0.001). There were no significant differences in the incidences of hypertensive disorder complicating pregnancy, gestational diabetes, hypothyroidism during pregnancy, premature rupture of membranes, placenta previa between the two groups (all P>0.05). The cesarean section rate, premature delivery rate, low birth weight rate, macrogenesis rate, sex ratio, neonatal body mass, neonatal length and neonatal birth defects rate were not significantly different between the two groups (all P>0.05). Conclusion:Trophoectoderm biopsy of blastocysts before FET does not affect serum β-hCG level in early pregnancy, and does not increase the risk of maternal and infant adverse complications.
