Effect of blastulation time on maternal and neonatal outcomes following single euploid blastocyst transfer
10.3760/cma.j.cn101441-20221130-00533
- VernacularTitle:整倍体囊胚发育天数对母婴结局的影响
- Author:
Ziyin DING
1
;
Jiaan HUANG
1
;
Yao LU
1
;
Yun SUN
1
Author Information
1. 上海交通大学医学院附属仁济医院生殖医学中心 上海市辅助生殖与优生重点实验室，上海 200135
- Publication Type:Journal Article
- Keywords:
Blastocyst transfer;
Pregnancy complications;
Neonatal outcomes;
Preimplantation genetic testing for aneuploidies;
Timing of blastulation
- From:
Chinese Journal of Reproduction and Contraception
2023;43(6):566-572
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether blastulation time would impact maternal and neonatal outcomes following single euploid blastocyst transfer.Methods:A retrospective cohort study was conducted at the Centre for Reproductive Medicine of Renji Hospital, Shanghai Jiao Tong University School of Medicine between October 2017 and June 2021. Totally 893 patients who received preimplantation genetic testing for aneuploidies (PGT-A) and delivered singleton live birth after frozen single euploid blastocyst transfer were included. Patients were divided into day 5 (D5) group ( n=768) and day 6 (D6) group ( n=125) according to timing of blastulation. Propensity score matching (PSM) with a ratio of 2∶1 was conducted to balance potential confounding factors including female age, body mass index (BMI) and infertility duration. After PSM, there were 250 patients in D5 group and 125 patients in D6 group. Basic characteristics, ovarian stimulation and embryo transfer cycle characteristics, pregnancy complications and neonatal outcomes of the two groups were compared. Furthermore, patients were divided into <35 years old group and ≥35 years old group by maternal age to conduct stratified analysis. Results:There were no statistically significant differences in the baseline characteristics including female age, BMI, infertility duration and indication for infertility treatment between the two groups after PSM (all P>0.05). The number of oocytes retrieved and the number of two pronuclei (2PN) did not differ between the two groups (all P>0.05), while the number of embryos cryopreserved was significantly increased in D5 group than in D6 group [5.00 (3.00, 7.00) vs. 3.00 (2.00, 5.00), P<0.001]. The proportion of cycles transferring good-quality embryos was significantly higher in D5 group than in D6 group [81.6% (204/250) vs. 40.0% (50/125), P<0.001]. No significant differences were observed in the incidences of gestational diabetes, hypertensive disorders of pregnancy, thyroid diseases during pregnancy, premature preterm rupture of membranes, preterm delivery, placenta disorder and postpartum hemorrhage between the two groups (all P>0.05). The birth weight, gestational age, neonate sex, the incidences of low birth weight, macrosomia, small for gestational age, large for gestational age and birth defects did not differ as well (all P>0.05). In stratified analysis, there were no significant differences in maternal and neonatal outcomes between D5 group and D6 group for women who aged <35 years or ≥35 years (all P>0.05). Conclusion:Timing of blastulation in euploid blastocysts does not impact maternal and neonatal outcomes.
