Differential expression of non-coding small RNA in spermatozoa with different DNA fragmentation index
10.3760/cma.j.cn101441-20221226-00590
- VernacularTitle:非编码小RNA在不同DNA碎片化指数的精子细胞中的表达差异
- Author:
Xibo WANG
1
;
Jian XU
;
Hongli YAN
;
Daru LU
Author Information
1. 复旦大学生命科学学院，上海 200438
- Publication Type:Journal Article
- Keywords:
Infertility,male;
Non-coding small RNA;
Sperm DNA fragmentation index;
Semen parameters
- From:
Chinese Journal of Reproduction and Contraception
2023;43(9):932-938
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression differences of small non-coding RNAs (sncRNAs) in sperm cells with different DNA fragmentation index (DFI).Methods:Male patients who visited the Center of Reproductive Medicine of the Changhai Hospital Affiliated to Naval Medical University from October 2021 to August 2022 were included in the study. According to the DFI value, they were divided into <15%, 15%-30% and >30%, which were denoted as normal DFI group ( n=48), critical DFI group ( n=40) and increased DFI group ( n=48). The relative expression levels of sncRNAs in sperm cells of 3 groups were compared. The correlation between differential sncRNAs and sperm motility was analyzed. Results:There were no significant differences in age and sperm concentration among the three groups (all P>0.05). The sperm motility in the increased DFI group [(30.36±4.75)%] was lower than that in the normal DFI group [(63.38±9.56)%, P<0.001] and the critical DFI group [(56.50±5.87)%, P=0.034]. Compared with the other two groups, the relative expression levels of Glu-CTC-40-10 and SeC-TCA-37-4 in the increased DFI group decreased, while the relative expression levels of Gly-GCC, iMet-CAT-18-18, and hsa-miR-151a-5p increased, with statistical significances (all P<0.001). Spearman correlation analysis showed that Glu-CTC-40-10, SeC-TCA-37-4 were positively correlated with sperm motility ( r=0.384, P<0.001; r=0.441, P<0.001), and Gly-GCC, iMet-CAT-18-18 and hsa-miR-151a-5p were negatively correlated with sperm motility ( r=-0.437, P<0.001; r=-0.423, P<0.001; r=-0.515, P<0.001). Conclusion:Compared with semen with normal DFI, sncRNAs are differentially expressed in semen with critical and elevated DFI, which may be related to the molecular mechanism of DFI formation. SncRNAs have the potential to be used as biological markers of male infertility.
