Establishement and ethical optimization of rat oral mucosa ulcer model
10.3969/j.issn.1001-3733.2025.01.004
- VernacularTitle:大鼠口腔溃疡模型构建与伦理优化研究
- Author:
Xiang LI
1
;
Kaiyan WANG
;
Weiwei YU
;
Xinyi HAO
;
Ling LI
;
Jianhong DUAN
;
Bin FENG
;
Qing LIU
;
Lingyun XIA
;
Lina NIU
Author Information
1. 442000 十堰,湖北医药学院附属太和医院口腔医学中心
- Publication Type:Journal Article
- Keywords:
Oral ulcer;
Animal model;
Ethical care
- From:
Journal of Practical Stomatology
2025;41(1):26-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop rat models of oral mucosa ulcer using distinct experimental methodologies,fulfilling research requirements and adhering to the ethical standards for animal care.Methods:96 SD rats were randomly allocated into groups.The rats in control group(n=8)were regularly fed without other treatment.Those in chemical cauterization groups were treated by 20％,40％,60％of glacial acetic acid(GAA)on oral mucosa(n=8);in mechanical damage groups by 30 000 r/min high speed drill induced trauma of 10,20 and 30 mm2 respectively(n=8);in ionizing radiation groups were treated with 10,12,15,20 and 30 Gy on the mucosa respectively(n=8).After the ulcer was appeared,the morphology of the mucosa were observed,the mucosal tissue lesions were examined by HE,Masson and immunofluorescence staining,the expression of TNF-α and IL-1β were detected by qPCR and ELISA respectively,and the body conditions such as diet and body weight of the rats were observed,the pain,dis-tress and discomfor of the rats were evaluated by MORTON&Griffits Guidelines.Results:40％and 60％GAA,20 mm2 and 30 mm2 friction damage and ionizing radiation of 12 Gy or greater may induce oral mucosa ulcer with a diseas coruse of 6-7 d in SD rats.TNF-α and IL-1β mRNA expression in the damaged tissue,the related protein expression in blood serum of the rats were in-creased.MORTON&Griffits Guidelines analysis showed 40％GAA,20 mm2 friction damage and 12 Gy ionizing radiation induced the lowest scores of pain,distress and discomfort of the rats with compatible oral mocosa ulcere induced by the relevat treatment.Conclusion:40％GAA,20 mm2 of friction damage and 12 Gy of ionizing radiation can reliably establish oral mucosa ulcer models and minimize adverse effects on SD rats,and accord with ethical standards of 3R for laboratory animal.
