Microbial community of root apical region with persistent apical periodontitis
10.3969/j.issn.1001-3733.2025.01.015
- VernacularTitle:持续性根尖周炎患牙根尖区微生物群落分析研究
- Author:
Xiaoqiang SUN
1
;
Zi YANG
;
Yong NIE
;
Benxiang HOU
Author Information
1. 100069 北京,首都医科大学附属北京口腔医院牙体牙髓科
- Publication Type:Journal Article
- Keywords:
Persistent apical periodontitis;
Microscopic apical surgery;
Root apical region;
Microbial community;
High-throughput sequencing
- From:
Journal of Practical Stomatology
2025;41(1):93-97
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To characterize the bacterial communities present in the root apical regions associated with persistent apical periodontitis(PAP).Methods:6 periapical lesion samples were collected from the teeth with PAP.DNA was extracted and ampli-fied by PCR based on the V3-V4 region of 16S rRNA gene.Illumina high throughput sequencing was applied to achieve the infor-mation of microbial community.Results:In total,447 OTUs were assigned,the average number of OTUs for the samples was 164.83±56.24.The phyla Firmicutes,Actinobacteria,Bacteroidetes,Proteobacteria and Fusobacteria were detected with high rela-tive abundances in all samples.At the genus level,the prevalent bacterial genera were Bifidobacterium,Streptococcus,Bacillus,En-terococcus and Porphyromonas,which were abundantly detected in all samples.The 6 samples were divided into 2 groups by hierar-chical cluster analysis.Prevotella and Treponema were abundant in group A,Bifidobacterium,Streptococcus and Enterococcus were abundant in group B.Conclusion:The bacterial profiles of root apical region of the teeth with PAP can be illustrated that diverse bacterial communities located in apical lesions.The bacterial composition of PAP can be divided into 2 microbial pattern and the re-lationship between different bacteria may guide new strategies to control infection.
