The influence of titanium nanotopography on the mitochondrial metabolism and polarization of macro-phages
10.3969/j.issn.1001-3733.2025.01.005
- VernacularTitle:钛表面纳米形貌对巨噬细胞线粒体代谢及极化方向影响的研究
- Author:
Meiqi ZHAO
1
;
Weihua YU
;
Li DING
;
Yan ZHANG
;
Wen SONG
;
Yumei ZHANG
Author Information
1. 710032 西安,口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔医学重点实验室,空军军医大学第三附属医院
- Publication Type:Journal Article
- Keywords:
Macrophage polarization;
Titanium dioxide nanotube arrays;
Mitochondria;
Metabolism reprogramming
- From:
Journal of Practical Stomatology
2025;41(1):34-40
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of different titanium(Ti)surfaces on mitochondria metabolism and polarization of mac-rophages.Methods:The anodic oxidation method prepared nanotubes with distinct morphologies(NT5 and NT20 groups)on smooth titanium surfaces(P group)were characterized by scanning electron microscopy(SEM).After 24 hours of culturing RAW264.7 cells on the samples,SEM was employed to observe the morphology of the cells of all groups.Levels of mitochondria ATP and ROS,as well as the activity of hexokinase(HK),were measured by fluorescence probes and luciferase assays.The expression of iNOS,Arg-1,TNF-α and CD206 mRNA was evaluated by RT-qPCR.Changes in mitochondria metabolism and macrophage polarization were studied following inhibition of cellular glycolysis by using 2-deoxy-D-glucose(2-DG)in groups P*and NT20*.Results:Compared with the P group,the NT5 group showed no significant changes in mitochondria ATP,ROS levels and HK activity,but an upregula-tion in M2-associated gene expression was observed.In the NT20 group,mitochondria ATP and ROS levels were increased,HK ac-tivity was elevated,and M1-associated gene expression was upregulated.After glycolysis inhibition,there was no significant differ-ence in mitochondria ATP,ROS levels,HK activity and polarization-related gene expression levels between the P*and NT20*groups compared with the P group.Conclusion:Large-diameter nanotubes may promote macrophage M1 polarization by regulating the metabolic reprogramming that occurs in macrophage mitochondria.
