Effect of simulated altitude of 6 500 m hypoxia environment on reproductive function of female mice
10.3760/cma.j.cn101441-20220808-00337
- VernacularTitle:模拟海拔6 500米低氧环境对雌性小鼠生殖功能的影响
- Author:
Wenjie PU
1
;
Wei MA
;
Juanwen MA
;
Ling WANG
Author Information
1. 中国人民解放军联勤保障部队第940医院生殖中心，兰州 730050
- Publication Type:Journal Article
- Keywords:
Plateau;
Female mice;
Hypoxia-inducible factor 1α;
Reproductive functions
- From:
Chinese Journal of Reproduction and Contraception
2023;43(11):1140-1145
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of simulated 6 500 m altitude hypoxia on the fertility of BALB/c female mice.Methods:In this study, 50 6-week-old BALB/c female mice were randomly divided into control group and plateau groups according to body weight. The plateau group divided into plateau 3-day group,plateau 5-day group,plateau 10-day group and plateau 15-day group, every group included 10 female mice. A low pressure hypoxic animal test chamber was used to establish a simulated low pressure hypoxia mouse model at an altitude of 6 500 m, the daily light/dark ratio was 12 h∶12 h. In each group, the hypoxia-inducible factor 1α (HIF-1α) level in the mouse ovary was tested by Western blotting (WB) and immunohistochemistry (IHC), mouse ovarian tissue morphology and the changes in the numbers of the egg follicles were observed by hematoxylion and eosin (HE) staining pathological section, enzyme-linked immunosorbent assay (ELISA) was used to get the serum values of reproductive hormone.Results:1) Compared with control group [(3 417.67±316.02) μg/L], the levels of anti-Müllerian hormone (AMH) decreased in all groups of the plateau, among them, plateau 10-day group [(2 643.50±210.01) μg/L], the plateau 5-day group [(2 195.50±160.88) μg/L] and plateau 15-day group [(2 174.40±709.23) μg/L] showed significant differences (all P<0.05). Compared with control group [(68.02±2.80) U/L], the levels of follicle-stimulating hormone (FSH) decreased in the plateau 5-day group [(64.20±4.11) U/L, P=0.029], plateau 10-day group [(59.05±5.16) U/L, P<0.001] and plateau 15-day group [(57.46±3.58) U/L, P<0.001]. Compared with control group [(6.71±1.01) U/L], the levels of luteinizing hormone (LH) decreased in the plateau 5-day group, plateau 10-day group and plateau 15-day group, among them, the plateau 10-day group [(5.18±0.30) U/L] and plateau 15-day group [(4.86±0.53) U/L] showed significant differences compared with control group ( P=0.005, P<0.001). Compared with control group [(105.56±5.43) pmol/L], the levels of estradiol in the plateau 5-day group [(90.92±5.08) pmol/L] and the plateau 15-day group [(81.46±3.96) pmol/L] showed significant differences compared with control group ( P=0.038, P<0.001). Compared with control group[(13.63±0.69) μg/L], the levels of progesterone in plateau groups were decreased, the plateau 3-day group [(11.45±0.65) μg/L], the plateau 5-day group [(8.86±0.72) μg/L], plateau 10-day group [(9.56±0.53) μg/L] and plateau 15-day group [(9.29±0.64) μg/L] showed significant differences compared with control group (all P<0.001). 2) Compared with control group, the numbers of ovarian follicles decreased, atretic follicles increased, granulosa cells apoptosis and venous vascular expansion were observed. 3) IHC showed that the ratio of HIF-1α positive cells increased with the extension of hypoxia. WB showed that the expressions of HIF-1α in the plateau groups increased compared with control group (all P<0.001). 4) Compared with control group (0.408±0.083), the ovarian index were decreased in plateau 3-day group (0.271±0.087), plateau 5-day group (0.114±0.035), plateau 10-day group (0.076±0.052) and plateau 15-day group (0.109±0.026), the difference was significant among the five groups ( P<0.001). Conclusion:Exposure to the simulated low oxygen environment of 6 500 m altitude makes the mouse ovarian tissue hypoxia, which causes certain adverse effects on the fertility of female mice, and provides a theoretical basis for the next research on female fertility protection.
