NMRAL1 promotes proliferation and migration of papillary thyroid carcinoma cells
10.13315/j.cnki.cjcep.2025.01.004
- VernacularTitle:NMRAL1促进甲状腺癌细胞的增殖和迁移
- Author:
Yiting XIE
1
;
Xinyi LONG
;
Shiyu CAO
;
Lin XIAO
;
Tengyun MA
;
Feng YE
Author Information
1. 四川大学华西医院病理科,成都 610041;四川大学临床病理研究所,成都 610041;四川大学临床医学院,成都 610041;四川大学疾病分子网络前沿医学中心移植免疫实验室,成都 610041
- Publication Type:Journal Article
- Keywords:
thyroid neoplasm;
NMRAL1;
proliferation;
migration;
translesion DNA synthesis
- From:
Chinese Journal of Clinical and Experimental Pathology
2025;41(1):14-22
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To explore the role of translesion synthesis-related gene NmrA like redox sensor 1(NMRAL1)in the development of papillary thyroid carcinoma(PTC).Methods Bioinformatics analysis was em-ployed to investigate the expression of the NMRAL1 gene across various cancer types and its correlation with prognosis.Immunohistochemistry was utilized to analyze the expression pattern of NMRAL1 in PTC and conduct clinicopathological correlation analysis.Additionally,stable cell lines overexpressing NMRAL1 were established in TPC-1 cells.The effects of NMRAL1 overexpression on cell proliferation,migration,and invasion of TPC-1 and Nthy-ori 3-1 cells were assessed using CCK-8 assay,clonogenic assay,Transwell assay,and scratch assay,respectively.Furthermore,RT-qPCR experiments were conducted to investigate the regulatory effect of NMRAL1 on the expression of genes associated with translesion DNA synthesis.Results Bioinformatics analysis revealed that NMRAL1 was highly expressed in all 26 types of tumors compared to normal tissues in the TCGA x GTEx dataset.Patients with high expression of NMRAL1 in thyroid cancer had significantly lower survival rates than those with low expression(P＜0.05).In the immunohisto-chemical validation of 30 PTC samples,NMRAL1 had a positive rate of 73.33％,in PTC tissues,while it was not ex-pressed in adjacent normal thyroid tissues.The staining intensity scores of NMRAL1 in PTC and adjacent tissues exhib-it significant differences(P＜0.000 1).High expression of NMRAL1 was associated with the size of the tumor(P=0.027 4)and lymph node metastasis(P=0.044 1).In vitro functional experiments revealed a significant enhance-ment in both cell proliferation and migration/invasion capacities upon overexpression of NMRAL1(P＜0.05).Moreo-ver,mRNA and protein expression levels of translesion synthesis-related genes were upregulated(P＜0.001).Con-clusion NMRAL1 promotes proliferation and migration of thyroid carcinoma cells,suggesting that this function may be achieved by enhancing DNA damage repair capacity,thereby improving the survival of damaged cells.
