Effects of carnosine on mouse oocyte in vitro maturation and embryo development
10.3760/cma.j.cn101441-20220922-00409
- VernacularTitle:肌肽对小鼠卵母细胞体外成熟和胚胎发育的影响
- Author:
Yun BAO
1
;
Keda YU
1
;
Juan CHAI
1
;
Jiating MAO
1
;
Shuai SHI
1
Author Information
1. 金华市人民医院生殖医学中心，金华 321000
- Publication Type:Journal Article
- Keywords:
Carnosine;
Embryo development;
Mitochondria;
Immature oocytes;
In vitro maturation
- From:
Chinese Journal of Reproduction and Contraception
2023;43(11):1146-1152
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of carnosine on in vitro maturation (IVM) of mouse oocytes and the developmental competency of embryos. Methods:The mouse cumulus-oocyte complexes (COC) were isolated and cultured in the IVM medium containing 10 μmol/L, 30 μmol/L, 50 μmol/L,100 μmol/L,or 200 μmol/L carnosine for 18 h. IVM medium without carnosine was acted as control group. The optimal concentration was chosen according to the rates of oocyte maturation, embryo cleavage and blastocyst formation. The effect of carnosine was further verified by detecting the levels of reactive oxygen species (ROS), and total glutathione (T-GSH), the distribution of cortical granules and mitochondria, and also the copy numbers of mitochondria.Results:In the 100 μmol/L carnosine group, the rates of oocyte maturation [69.23% (135/195)], embryo cleavage [66.06% (72/109)] and blastocyst formation [48.61% (35/72)] were the highest and showed statistically significant differences ( P<0.001, P<0.001 , P=0.010) when compared with control group [44.44% (84/189), 38.67% (29/75), 20.69% (6/29)]. Therefore, 100 μmol/L was selected as the optimal concentration of carnosine. The levels of ROS and T-GSH in control group were 2.04 and 0.64 times as those in the 100 μmol/L carnosine group (both P<0.001). The percentage of grade Ⅲ cortical granules in the 100 μmol/L carnosine group was significantly higher than that in control group ( P<0.001). The mitochondria of oocytes in control group were uniformly distributed, while the mitochondria in the 100 μmol/L carnosine group gathered in the central cytoplasmic region, and the fluorescence intensity was higher than that in control group ( P=0.040). The mitochondrial copy number in the 100 μmol/L carnosine group was as much 1.72 times as that in control group ( P<0.001). Conclusion:Carnosine effectively improves oxidative stress in the immature oocytes and promotes the mouse oocyte IVM and embryo development.
