Effect of LINC00839 on the malignant biological behavior of endometrial cancer cells by regulating the miR-625-5p/MSI1 axis
10.12007/j.issn.0258-4646.2025.02.005
- VernacularTitle:LINC00839调节miR-625-5p/MSI1轴对子宫内膜癌细胞恶性生物学行为的影响
- Author:
Ji HUANG
1
;
Xiujuan DENG
1
;
Xian CHENG
1
Author Information
1. 江西省九江市妇幼保健院妇科,江西九江 332000
- Publication Type:Journal Article
- Keywords:
endometrial cancer;
malignant biological behavior;
long non-coding RNA;
LINC00839;
miR-625-5p;
MSI1
- From:
Journal of China Medical University
2025;54(2):121-126,132
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of long non-coding RNA,LINC00839,on the malignant biological behavior of endome-trial cancer(EC)cells via regulating miR-625-5p/MSI1 axis.Methods The expression of LINC00839,miR-625-5p,and MSI1 mRNA in EC tissues and cells was detected by real-time quantitative PCR.Ishikawa cells were selected,and bioinformatics,dual-luciferase reporter gene assay,and RNA-binding protein immunoprecipitation assay were performed to verify the targeting relationship between LINC00839,MSI1,and miR-625-5p.CCK-8,colony formation assay,flow cytometry,and Transwell assay were performed to detect cell proliferation,apoptosis,migration,and invasion.Western blotting was used to detect the expression of MSI1,Bcl-2,Bax,MMP-2,and MMP-9 protein.In vivo tumor formation experiments were conducted to verify the effect of LINC00839 on transplanted tumors in nude mice.Results The expression of LINC00839 and MSI1 mRNA in EC tissues was higher,whereas the expression of miR-625-5p was lower(P＜0.05).LINC00839 and MSI1 targeted miR-625-5p.LINC00839 knockdown or miR-625-5p overexpression suppressed malignant behavior of cells(P＜0.05).Inhibition of miR-625-5p expression or overexpression of MSI1 reversed the inhibitory effect of LINC00839 knockdown or miR-625-5p overexpression on the malignant behavior of cells(P＜0.05).LINC00839 knockdown decreased the volume and mass of transplanted tumors,increased the expression of miR-625-5p,and inhibited the expression of MSI1.Conclusion LINC00839 can target the miR-625-5p/MSI1 axis and regulate the proliferation,apoptosis,migration,and invasion of EC cells.
