Effects and mechanism of platelet-rich plasma combined with periodic mechanical stretching on C2C12 myotube atrophy
10.3969/j.issn.1001-1242.2025.01.003
- VernacularTitle:富血小板血浆结合周期性机械牵拉对C2C12肌管萎缩的影响及机制探讨
- Author:
Xiaoting XIE
1
;
Yong HE
;
Yantao MA
Author Information
1. 复旦大学附属华东医院疼痛科上海市老年医学临床重点实验室,上海市,200040;上海体育大学运动健康学院
- Publication Type:Journal Article
- Keywords:
platelet-rich plasma;
C2C12 skeletal muscle cells;
periodic mechanical stretch;
differentiation;
mus-cle atrophy;
sarcopenia
- From:
Chinese Journal of Rehabilitation Medicine
2025;40(1):8-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study effects and mechanism of platelet-rich plasma combined with periodic mechanical stretch on C2C12 myotube atrophy.Method:The expression of MyoD and MyoG was detected by Westem Blot(WB)to confirm the optimal con-centration of PRP;Subsequently,the pro-differentiation effect of PRP and periodic mechanical stretch(Str)on C2C12 cells was explored;C2C12 cells were induced to shrink by TNF-α,and the models were verified by Modified Giemsa staining.To explore the effect and mechanism of 2％PRP combined with periodic mechanical stretching in improving the atrophy of C2C12 cells,the experiments were divided into control group,TNF-αgroup,TNF-α+2％PRP group,TNF-α+2％PRP+Str group.The changes of MuRF-1,Fbx32 and Akt,p-Akt,p70 and p-p70 were detected by adding the inhibitors.Result:The expression levels of MyoD and MyoG in the 2％PRP group and the 2％PRP+Str group were significantly increased.In the anti-atrophy experiment,the modified Giemsa staining showed the myotubes in the TNF-α group were significantly atrophied,and the TNF-α+2％PRP group can improve myotube atrophy.The expressions of MuRF-l and Fbx32 were decreased in the TNF-α+2％PRP+Str group.After GDC0068 inter-vention,the anti-atrophic effect of PRP combined with periodic mechanical stretch was inhibited.Conclusion:2％PRP combined with periodic mechanical stretch can effectively promote the differentiation of C2C12 cells,improve myotube atrophy,and play a role through the Akt/p70 signaling pathway.
