Deciphering the protective role of AZGP1 in heart failure through Mendelian randomization
10.3969/j.issn.1674-8115.2025.08.011
- VernacularTitle:孟德尔随机化解析AZGP1在心力衰竭中的保护作用
- Author:
Long LI
1
;
Xia ZHAO
;
Shan JIN
;
Zeying LI
;
Fuqiang LÜ
;
Lijuan PANG
;
Kejian LIU
Author Information
1. 石河子大学第一附属医院,国家卫生健康委员会中亚高发病防治重点实验室,石河子 832000;广东省湛江中心人民医院病理科,湛江 524000
- Publication Type:Journal Article
- Keywords:
heart failure;
AZGP1;
plasma protein;
Mendelian randomization;
biomarker
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2025;45(8):1035-1045
- CountryChina
- Language:Chinese
-
Abstract:
Objective·To investigate the causal relationship between plasma zinc-alpha-2-glycoprotein 1(AZGP1)and heart failure(HF)by using Mendelian randomization(MR)analysis and experimental validation.Methods·A two-sample MR analysis was performed to assess the causal relationship between AZGP1 and HF by integrating large-scale genome-wide association study(GWAS)data on plasma proteins and HF.The inverse-variance weighted(IVW)method was employed as the primary analytical approach,supplemented by MR-Egger regression,weighted median,and simple median methods.Horizontal pleiotropy was tested by using MR-PRESSO global test and MR-Egger intercept analysis.Colocalization analysis was conducted to validate genetic locus overlap.Additionally,a clinical cohort(84 HF patients and 68 healthy controls)was analyzed,with plasma AZGP1 levels quantified by enzyme-linked immunosorbent assay(ELISA).Results·MR analysis showed that elevated plasma AZGP1 levels were significantly associated with reduced HF risk(OR=0.82,95%CI 0.75?0.90,P=1.70×10-5).Colocalization analysis confirmed that AZGP1 expression and HF shared causal genetic variants(posterior probability for H4=0.69).Sensitivity and reverse MR analyses supported the robustness of the results.ELISA confirmed that plasma AZGP1 levels were significantly lower in HF patients compared to healthy controls,reinforcing its protective role in HF.Conclusion·This study demonstrates AZGP1 exerts a protective causal effect on HF and may serve as a potential biomarker for HF treatment.
