Effect of euploid embryo morphokinetic parameters on pregnancy outcome of single blastocyst transfer
10.3760/cma.j.cn101441-20220813-00348
- VernacularTitle:整倍体胚胎形态动力学参数对单囊胚移植妊娠结局的影响
- Author:
Jiahong ZHU
1
;
Shun XIONG
1
;
Lihong WU
1
;
Wei HAN
1
;
Guoning HUANG
1
;
Junxia LIU
1
Author Information
1. 重庆市妇幼保健院人类胚胎工程重庆市重点实验室 重庆医科大学附属妇女儿童医院生殖医学中心，重庆 400013
- Publication Type:Journal Article
- Keywords:
Pregnancy outcome;
Morphokinetics;
Euploid blastocyst;
Embryo quality
- From:
Chinese Journal of Reproduction and Contraception
2023;43(9):898-903
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of morphokinetic parameters during embryo development on pregnancy outcome of euploid single blastocyst transfer cycles, and to evaluate the predictive value of morphokinetic parameters for selective euploid single blastocyst transfer strategy.Methods:A retrospective cohort study was conducted to analyze the clinical data of 394 patients who received preimplantation genetic testing-frozen-thawed embryo transfer (PGT-FET) in Center for Reproductive Medicine of Women and Children's Hospital of Chongqing Medical University from January 2019 to June 2021. According to the pregnancy outcomes, the patients were divided into non-implantation group ( n=153), clinical miscarriage group ( n=16) and live birth group ( n=225). The patients' baseline characteristics, embryo quality and morphokinetic parameters were compared among the three groups. Results:The maternal age in the non-implantation group [(32.51±4.08) years] was significantly higher than that in the live birth group [(31.34±4.23) years, P=0.025], and the proportion of high-quality embryos in the non-implantation group [26.80% (41/153)] was significantly lower than that in the live birth group [42.22% (95/225), P=0.007]. There were no significant differences in the body mass index and the proportion of high-quality embryos between the clinical miscarriage group and the non-implantation group ( P>0.05). There was no significant difference in the proportion of high-quality embryos between the clinical miscarriage group and the non-implantation group ( P>0.05). There were no significant differences in the morphokinetic parameters of time to pronuclei appearance (tPNa), time to PN fading (tPNf), time to 2-cell (t2), t3, t4 and t8 among the three groups (all P>0.05). The average timing of compacted morula (tM) [(86.96±7.59) h] and timing of starting blastulation (tSB) [(96.73±7.20) h] of embryos in the non-implantation group were significantly higher than those in the live birth group [(85.00±7.00) h, P=0.010; (95.14±7.30) h, P=0.037, respectively] and the clinical miscarriage group [(82.89±6.33) h, P=0.040; (93.02±6.10) h, P=0.048]. After adjusting for age and embryo quality, the regression analysis showed that the morphokinetic parameters had no significant effect on implantation results (all P>0.05). Conclusion:The morphokinetic parameters during embryonic development do not affect the pregnancy outcome of euploid single blastocyst transfer and cannot be used to predict the pregnancy outcome of euploid blastocyst transfer.
