Effect of oxidative stress on the quality of oocytes and embryos in patients with polycystic ovary syndrome
10.3760/cma.j.cn101441-20220331-00137
- VernacularTitle:多囊卵巢综合征患者氧化应激水平对卵子和胚胎质量的影响
- Author:
Lin ZHANG
1
;
Ling ZHANG
;
Limei WU
;
Weihai XU
;
Zhen JIN
;
Fan JIN
Author Information
1. 浙江大学医学院附属妇产科医院生殖内分泌科，杭州 310005
- Publication Type:Journal Article
- Keywords:
Fertilization in vitro;
Oxidative stress;
Polycystic ovary syndrome;
Embryonic development
- From:
Chinese Journal of Reproduction and Contraception
2023;43(7):690-696
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship of oxidative stress levels in serum and follicular fluid of polycystic ovary syndrome (PCOS) patients with the quality of oocytes and embryos.Methods:A prospective follow-up study was conducted to analyze 67 patients with PCOS (PCOS group) and 66 infertility patients with simple fallopian tube factors (control group) who underwent in vitro fertilization in Center for Reproductive Medicine, Zhejiang Provincial People's Hospital from January 2019 to October 2020. Serum and follicular fluid from the follicles with a diameter of more than 18 mm were collected on the oocyte retrieval day. Colorimetric method was used to detect the levels of total antioxidant activity (TAC), superoxide dismutase (SOD), and malondialdehyde (MDA), and the differences of those oxidative stress indicators between the two groups were compared and the correlation of those parameters with oocyte quality, embryo development and clinical outcomes were analyzed. Results:The serum SOD level of the PCOS group [(22.11±3.70) U/mL] was significantly lower than that of control group [(25.70±3.32) U/mL, P<0.001]. The follicular fluid TAC level [(0.62±0.05) mmol/L], MDA level [(16.64±3.85) nmol/mL] and serum MDA level [(18.20±4.68) nmol/mL] of the PCOS group were significantly higher than those of control group [(0.53±0.04) mmol/L, P<0.001; (13.74±2.28) nmol/mL, P<0.001; (15.37±5.34) nmol/mL, P=0.008]. The good-quality embryo rate [34.1% (341/1 000)], the implantation rate [49.5% (54/109)], the clinical pregnancy rate [60.9% (39/64)] and the live birth rate [50.0% (32/64)] in the PCOS group were statistically different from those in control group [41.7% (306/733), P=0.013; 58.7% (54/92), P=0.023; 70.3% (45/64), P=0.006;59.4% (38/64), P=0.007]. There was a negative correlation between the TAC level in follicular fluid and the rate of good-quality embryo ( β=-1.83, P=0.012). There was no significant correlation between oxidative stress indicators and clinical outcomes. Conclusion:The TAC level of follicular fluid in PCOS patients is elevated, and increased TAC level in follicular fluid may be one of the risk factors for poor embryo quality.
