Influencing factors of polyspermy during conventional in vitro fertilization in super-long protocol
10.3760/cma.j.cn101441-20211108-00494
- VernacularTitle:超长方案常规体外受精多精受精发生的影响因素分析
- Author:
Yating SUN
1
;
Jia WANG
1
;
Xiaoxia LI
1
;
Aizhen ZHU
1
Author Information
1. 山西省运城市中心医院生殖医学科，运城 044000
- Publication Type:Journal Article
- Keywords:
Fertilization in vitro;
Polyspermy;
Influencing factors;
Super-long protocol
- From:
Chinese Journal of Reproduction and Contraception
2023;43(2):169-175
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate influencing factors of polyspermy during conventional in vitro fertilization (IVF) in super-long protocol. Methods:This study was a retrospective case-control analysis. From October 2018 to August 2021, 316 patients who underwent conventional IVF using super-long protocol in the Department of Reproductive Medicine, Yuncheng Central Hospital of Shanxi Province were selected and divided into normal fertilization group and polyspermy fertilization group according to polyspermy fertilization incidence in this cycle. The general data and clinical assisted pregnancy data in the two groups were analyzed. The correlation factors of P<0.15 in the single factor analysis were included in logistic regression model for multivariate analysis. Results:There were 227 polyspermy fertilization cycles out of 316 IVF cycles, and the incidence of polyspermy fertilization was 71.84%. There were no significant differences in female age, infertility duration, primary infertility ratio, infertility factors, body mass index (BMI), basal follicle-stimulating hormone (FSH) level and basal anti-Müllerian hormone (AMH) level between normal fertilization group and polyspermy fertilization group (all P>0.05). There were no significant differences in gonadotropin (Gn) initiation dosage, total dosage and duration of Gn used, and progesterone (P) on human chorionic gonadotropin (hCG) injection day between normal fertilization group and polyspermy fertilization group (all P>0.05). The number of follicles with a diameter of ≥14 mm, estradiol (E 2) on hCG injection day and the number of retrieved oocytes in polyspermy fertilization group were significantly higher than those in normal fertilization group [13.66±4.51 vs. 11.78±3.54, P<0.001; (17 247.49±9 450.74) pmol/L vs. (14 135.29±7 369.84) pmol/L, P=0.002; 17.48±7.37 vs. 13.01±6.17, P<0.001]. Luteinizing hormone (LH) level on hCG injection day in polyspermy fertilization group was significantly lower than that in normal fertilization group [(0.79±0.51) U/L vs. (1.02±0.78) U/L, P=0.014]. Logistic regression results showed that the number of follicles with a diameter of ≥14 mm, E 2 and P level on hCG injection day could not enter the regression equation ( OR=1.063, OR=1.676 and OR=1.078, respectively, all P>0.05); LH level on hCG injection day and the number of retrieved oocytes were correlated with polyspermy ( OR=1.900, P=0.028; OR=2.539, P=0.003). Conclusion:The incidence of polyspermy fertilization cycle during conventional IVF in super-long protocol was high. The number of follicles with a diameter of ≥14 mm, E 2 and LH levels on hCG injection day and the number of retrieved oocytes were significantly correlated with the occurrence of polyspermy. Lower LH level on hCG injection day and larger number of retrieved oocytes were independent risk factors of conventional IVF polyspermy fertilization in super-long protocol, and the number of retrieved oocytes had the greater influence on polyspermy fertilization.
