Role of resveratrol in regulating miR-125b-5p/PRDM1 axis in improving airway inflammation in children with asthma and its molecular mechanism
10.3969/j.issn.1000-484X.2025.08.018
- VernacularTitle:白藜芦醇调控miR-125b-5p/PRDM1轴在改善儿童哮喘气道炎症反应中的作用及其分子机制
- Author:
Dan SU
1
;
Yanan LIU
;
Lin ZHANG
Author Information
1. 衡水市人民医院,衡水 053000
- Publication Type:Journal Article
- Keywords:
Resveratrol;
miR-125b-5p;
PRDM1;
Children asthma;
Airway inflammation
- From:
Chinese Journal of Immunology
2025;41(8):1901-1907
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of resveratrol(RES)in improving airway inflammation in children with asthma by regulating miR-125b-5p/PRDM1 axis and its molecular mechanism.Methods:Rat model of asthma was induced by ovalbumin(OVA).The rats were randomly divided into sham group(sham),asthma group(model),10 μmol/L RES(RES-L)group,50 μmol/L RES(RES-H)group,50 μmol/L miR-125b-5p antagomir(miR-125b-5p antagomir)group and miR-125b-5p antagomir+50 μmol/L RES(miR-125b-5p antagomir+RES)group.The number of inflammatory cells in bronchoalveolar lavage fluid(BALF)was detected.HE staining and Masson three-color staining were used to observe pathological changes of lung tissue.Levels of inflammatory factors in lung homogenates were detected by ELISA.Serum from asthmatic children and healthy children was collected for analysis.Expressions of miR-125b-5p and PRDM1 in lung tissues of asthmatic and healthy children and different groups of rats were detected by RT-qPCR.Targeted binding relationship between miR-125b-5p and PRDM1 was detected by double luciferase assay.Results:HE staining showed that the airway epithelium in asthma group had a variety of inflammatory cell infiltration,while pathological changes in RES group were reduced.Masson staining showed that the area of airway collagen deposition was reduced in RES group compared with asth-ma group.The number of inflammatory cells in BALF in asthma group was higher than that in RES group.RES treatment significantly reduced airway wall and smooth muscle thickness.ELISA results showed that lung tissue in the asthma group had increased levels of IL-1β,IL-10 and TNF-α,while decreased level of IL-12,the RES group showed the opposite trend.Level of miR-125b-5p in lung tissue of asthmatic rats was significantly increased,while level of PRDM1 was decreased,and RES treatment could reverse this result.In addi-tion,treatment with miR-125b-5p antagomir alleviated airway inflammation and airway remodeling in asthmic rats.Dual luciferase assay confirmed the targeting binding relationship between miR-125b-5p and PRDM1.Compared with healthy children,serum levels of IL-1β,IL-10 and TNF-α were increased,while IL-12 level was decreased in children with asthma.Conclusion:RES inhibits the re-lease of inflammatory cytokines through HMGB1/TLR4/NF-κB pathway,alleviating asthma-induced airway inflammation and airway remodeling.
