Mechanism of Gegen Qinlian Decoction regulating PI3K/AKT/FOXO1 signaling pathway on anti-inflammatory and anti-oxidative stress in rats with non-alcoholic steatohepatitis
10.3969/j.issn.1000-484X.2025.08.017
- VernacularTitle:葛根芩连汤调控PI3K/AKT/FOXO1信号通路对非酒精性脂肪性肝炎大鼠抗炎和抗氧化应激的作用机制
- Author:
Chaoyun ZHANG
1
;
Na FEI
;
Pengfei HAO
;
Mengyu MU
;
Zhongming ZHANG
Author Information
1. 南阳理工学院张仲景国医国药学院,南阳 473004
- Publication Type:Journal Article
- Keywords:
Gegen Qinlian Decoction;
Non-alcoholic steatohepatitis;
Anti-inflammatory;
Anti-oxidative stress
- From:
Chinese Journal of Immunology
2025;41(8):1895-1900
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate influence of Gegen Qinlian Decoction(GQD)on anti-inflammatory,anti-oxidative stress activities and related pathways in rats with non-alcoholic steatohepatitis(NASH),and to clarify its mechanism.Methods:NASH rat model was established with methionine choline deficiency feed,and blank group,model group,positive group and different doses of GQD administration groups were set.By analyzing rat serum inflammation indicators,liver tissue antioxidant capacity indicators,liver tissue pathological sections,and combined with Western blot and RT-qPCR technology,anti-inflammatory and anti-oxidative stress mechanism of GQD on NASH rats were finally clarified.Results:Each dose of GQD could significantly reduce serum levels of IL-6,IL-8,IL-10,IFN-α and IFN-β in NASH model rats.GQD medium and high doses groups were statistically significant compared with model group and positive group;each dose of GQD could significantly reduce contents of ALT,AST and MDA in liver tissue of NASH rats,and significantly increase SOD,GPX,T-AOC contents at the same time;Western blot and RT-qPCR results showed that GQD could significantly reduce expressions of PI3K,AKT,p-FOXO1 protein and mRNA in liver tissue of NASH model,significantly increase expressions of FOXO1,SIRT1,Catalase protein and mRNA,and compared with positive group at high doses,it was also statistically significant.Conclusion:GQD can treat NASH by regulating PI3K/AKT/FOXO1 signaling pathway and exerting anti-inflammatory and anti-oxidative stress effects.
