Recent advances in analysis and application of TCR repertoire
10.3969/j.issn.1000-484X.2025.08.037
- VernacularTitle:TCR组库分析及应用的最新进展
- Author:
Zhihao FANG
1
;
Fangqi GONG
1
Author Information
1. 浙江大学医学院附属儿童医院心内科,国家儿童健康临床研究中心,杭州 310052
- Publication Type:Journal Article
- Keywords:
T cell receptor;
TCR repertoire;
Single-cell sequencing;
Antigen-specificity;
Application
- From:
Chinese Journal of Immunology
2025;41(8):2012-2018
- CountryChina
- Language:Chinese
-
Abstract:
Mature T cells can specifically recognize antigens presented on molecules of the major histocompatibility complex via their T cell receptors(TCR),which undergo unique molecular restructuring mechanisms.Thanks to the development of next-gener-ation sequencing technology and single-cell sequencing technology,high-throughput detection of TCR sequences can be used in T cell research.Descriptive analysis,assessment of repertoire diversity and clonal expansion,together with identification and detection of antigen-specific sequences are all valuable for measuring the TCR repertoire.TCR sequencing and repertoire analysis have contributed to research progress in related diseases in the fields of infection,tumor and immunology.Consequently,TCR repertoire analysis can be employed to assess disease progression and prognosis,it also has the potential to serve as a diagnostic biomarker for treatment.Furthermore,TCR repertoire analysis may also facilitate further advancement of T cell receptor engineered T cell therapy(TCR-T).This review provides a comprehensive overview of the recent advances in the analysis and application of TCR repertoire.
