The value of EBNA2 in distinguishing infectious mononucleosis from EBV-associ-ated diffuse large B-cell lymphoma
10.13315/j.cnki.cjcep.2025.08.003
- VernacularTitle:EBNA2在鉴别传染性单核细胞增多症和EBV相关弥漫性大B细胞淋巴瘤中的价值
- Author:
Jianlan XIE
1
;
Lujing YANG
1
;
Bing YUE
1
;
Yuanyuan ZHENG
1
;
Yanlin ZHANG
1
;
Xiaoge ZHOU
1
Author Information
1. 首都医科大学附属北京友谊医院病理科,北京 100050
- Publication Type:Journal Article
- Keywords:
infectious mononucleosis;
diffuse large B-cell lymphoma;
transplantation;
EBNA2
- From:
Chinese Journal of Clinical and Experimental Pathology
2025;41(8):991-996,1003
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To compare the expression and pattern of EBNA2 in infectious mononucleosis(IM),EBV-positive diffuse large B-cell lymphoma(EBV+DLBCL),and EBV+DLBCL arising in immune deficiency/dysregulation(IDD-related EBV+DLBCL),and to investigate the potential diagnostic value of EBNA2 in IM and EBV-associated diffuse large B-cell lymphoma.Methods A retrospective study was conducted on 46 cases of IM,31 cases of EBV+DLBCL,and 16 cases of IDD(post-transplantation)-related EBV+DLBCL.Clinical information,immunohistochemis-try and EBER were reviewed to further confirm the diagnoses.All samples were stained for EBNA2.The expression ra-tio and intensity of EBER and EBNA2 in the same area were assessed.Results EBER was positive in all IM,EBV+DLBCL,and IDD(post-transplantation)-related EBV+DLBCL,while the positivity rate of EBNA2 was 95.65％,6.45％,and 100％,respectively.The positive intensity of EBNA2 was weak(71.73％),strong(87.5％)and nega-tive(93.54％)in IM,IDD(post-transplantation)-related EBV+DLBCL and EBV+DLBCL respectively.The average values of EBNA2/EBER were 31％,3％,and 78％among the three groups.The positivity rate and average value of EBNA2/EBER in IM were significantly higher than those in EBV+DLBCL(P＜0.001);however,the average value of EBNA2/EBER was significantly lower than that in IDD(post-transplantation)-related EBV+DLBCL(P＜0.001).The weak positive expression of EBNA2 in IM was significantly higher than that in EBV+DLBCL(P＜0.001),where-as strong positive expression of EBNA2 in IDD(post-transplantation)-related EBV+DLBCL was higher than that in IM(P＜0.001).Conclusion EBNA2 is often positive in IM and predominantly weakly positive,which is distinct from the pattern in EBV+DLBCL and IDD(post-transplantation)-related EBV+DLBCL.EBNA2 can serve as an effective marker for distinguishing them.
