Serological and gene sequence analysis of rare Rhnull with immune antibody-Rh29

VernacularTitle:伴免疫性抗-Rh29的罕见Rhnull血清学和基因序列分析
Author: Qin YU ¹ ; Sheng CHEN ; Xinyi ZHANG ; Lin QU ; Fengxia LIU
Author Information

1. 张家界人民医院输血科,张家界 427000
Publication Type:Journal Article
Keywords: Rh; Novel RhAG mutation; Rhnull; Anti-Rh29
From: Chinese Journal of Immunology 2025;41(8):1985-1988,1996
CountryChina
Language:Chinese
Abstract: Objective:To investigate serological characteristics of Rhnull with anti-Rh29 and its rare gene sequence.Methods:Microcolumn gel method was used for ABO and RhCcDEe blood typing,accidental antibody identification and cross matching.RHD/RHCE genotype and sequencing analysis were performed by PCR-SSP method.RHAG gene was sequenced by fluorescence PCR method.Antigen or protein associated with Rh complex was detected by flow cytometry.Results:Blood group of the patient was A,Rh pheno-type was typed as group C-c-D-E-e,presented with symptoms of anemia,proportion of globular and oral erythrocytes in blood smear was 6.0%and 0.8%,respectively,RH genotype was CCDee,and immune anti-Rh29 high-frequency antigen were developed.RHAG gene sequencing revealed exons 8,9 and 10 deletion.Conclusion:Mechanism of"regulator"type Rhnull generation in this study is caused by deletion of exons 8,9 and 10 in RHAG gene.