Expression of miR-19a-3p in thymus of patients with myasthenia gravis and its effect on T cell proliferation
10.3969/j.issn.1000-484X.2025.08.031
- VernacularTitle:miR-19a-3p在重症肌无力患者胸腺中的表达及其对T细胞增殖的影响
- Author:
Lei SUN
1
;
Rui ZHAO
;
Qianru LI
;
Xiaoyan XUAN
;
Peng WANG
;
Ying DU
;
Pingping LIU
Author Information
1. 河南中医药大学第一附属医院体检中心,郑州 450000
- Publication Type:Journal Article
- Keywords:
Myasthenia gravis;
Thymus;
microRNA;
T cells;
Apoptosis
- From:
Chinese Journal of Immunology
2025;41(8):1980-1984
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of miRNAs in thymus of patients with myasthenia gravis(MG)and the re-lated mechanism of action,so as to provide theoretical and experimental basis for clinical diagnosis and treatment.Methods:miRNA microarray technology was used to analyze the differential miRNA expression in MG patients thymus.Fluorescence quantitative PCR and in situ hybridization were used to verify the expression of miR-19a-3p in thymus tissue.The TALL-104 cell line was transfected with miR-19a-3p mimics to observe the effects of miR-19a-3p on cell proliferation,apoptosis and expressions of related molecules(BCL2 and SOCS3).Results:Compared to normal thymus tissue,a total of 282 differentially expressed miRNAs were detected in the thymus of MG patients,among which 103 were up-regulated and 179 were down-regulated.The target genes of differentially expressed miRNAs were mainly related to nuclear molecules,cytoplasmic membrane-like structures and organelle related molecules.Quantita-tive fluorescence PCR and in situ fluorescence hybridization confirmed that the expression of miR-19a-3p in MG patients thymus was significantly lower than that in normal control group.Compared with the control group,miR-19a-3p mimics transfection could signifi-cantly inhibit the apoptosis of TALL-104 cells,increase the expression of BCL2 and decrease the expression of SOCS3(P<0.05).Conclusion:The expression of miRNA in the thymus of MG patients is significantly different from that of non-MG patients,and miR-19a-3p inhibits T cell apoptosis through up-regulation of BCL2 and down-regulation of SOCS3.
