Inhibition of testosterone on LPS-induced M1 macrophage polarization
10.3969/j.issn.1000-484X.2025.08.024
- VernacularTitle:睾酮抑制脂多糖诱导的巨噬细胞M1型极化
- Author:
Guoliang TIAN
1
;
Lidong ZHANG
1
;
Yundong XIA
1
;
Long YI
1
;
Jundong ZHU
1
;
Mantian MI
1
Author Information
1. 陆军军医大学暨第三军医大学军事预防医学系营养与食品安全研究中心,重庆市营养与食品安全重点实验室,重庆市医学营养研究中心,重庆 400038
- Publication Type:Journal Article
- Keywords:
Testosterone;
M1 polarization;
LPS;
Macrophage
- From:
Chinese Journal of Immunology
2025;41(8):1940-1944
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate effect of testosterone on polarization of M1 macrophages induced by lipopolysaccharide(LPS).Methods:CCK-8 method was used to detect effects of LPS and testosterone on RAW 264.7 cell viability.Morphological changes of cells were observed by optical microscope.mRNA expression levels of M1-type polarizing genes TNF-α,IL-1β and IL-6 in macro-phages were detected by qRT-PCR.Expression levels of M1-polarizing protein TNF-α and CD206 in macrophages were detected by immunofluorescence and Western blot.Secretion of inflammatory cytokines was detected by ELISA.Results:Testosterone could decrease mRNA expressions of TNF-α,IL-1β and IL-6 mRNA and protein expression of TNF-α.Finally,testosterone could decrease secretion of inflammation-related factors.Conclusion:Testosterone can inhibit LPS-induced transformation of macrophages to M1 polarization phenotype.
