Efficiency of "short time sampling" in noninvasive preimplantation genetic testing: a prospective study
10.3760/cma.j.cn101441-20220826-00359
- VernacularTitle:“短时取样法”用于非侵入性胚胎植入前遗传学检测效能的前瞻性研究
- Author:
Jiao LIN
1
;
Bing XIE
1
;
Jianping ZENG
1
;
Xiaojun TAN
1
;
Ke PENG
1
;
Zhenyu ZUO
1
;
Yankun HE
1
;
Xianghong HUANG
1
Author Information
1. 湘潭市中心医院生殖中心，湘潭 411100
- Publication Type:Journal Article
- Keywords:
Non-invasive preimplantation genetic testing;
Preimplantation genetic testing;
Short time sampling;
Next generation sequencing
- From:
Chinese Journal of Reproduction and Contraception
2023;43(8):825-829
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the efficiency of "short time sampling" for noninvasive preimplantation genetic testing (ni-PGT).Methods:A prospective study was conducted. The blastocysts of patients who were treated by preimplantation genetic testing for aneuploidy (PGT-A) in the Reproductive Center of Xiangtan Central Hospital from March 2021 to June 2022 were biopsied, meanwhile the spent culture medium (SCM) were collected. According to the different sampling methods for SCM, they were divided into two groups, direct sampling method and "short time sampling". The coincidence rate between ni-PGT and PGT-A was analyzed and compared statistically.Results:Totally 108 blastocysts from 36 couples were biopsied and SCMs were collected, including 59 cases of direct collection and 49 cases of "short time sampling". The area under curve (AUC) of direct collection method for diagnosing embryo ploidy was lower than that of "short time sampling" (0.628 vs. 0.785). Conclusion:The efficiency of "short time sampling" in diagnosing embryo ploidy is better than that of direct collection method.
