Preliminary study of fertility preservation by oocytes retrieval during gynecological operation
10.3760/cma.j.cn101441-20221009-00440
- VernacularTitle:妇科手术过程中取卵行生育力保存的初步研究
- Author:
Ruirui PENG
1
;
Ran DONG
1
;
Miao LI
1
;
Shuai ZHANG
1
;
Ting HAN
1
;
Yuzhen HUANG
1
;
Mei LI
1
Author Information
1. 山东大学生殖医学研究中心（山东大学附属生殖医院）生殖内分泌教育部重点实验室（山东大学）山东省生殖医学重点实验室 山东省生殖健康临床医学研究中心 山东省生殖健康技术创新中心 国家辅助生殖与优生工程技术研究中心，济南 250012
- Publication Type:Journal Article
- Keywords:
Laparoscopy;
Neoplasms;
Gynecological operation;
Follicle puncture;
Oocyte in vitro maturation;
Fertility preservation
- From:
Chinese Journal of Reproduction and Contraception
2023;43(2):145-149
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To find a method for fertility preservation through oocytes retrieval during gynecological operation and provide an experimental basis for patients who are not suitable for fertility preservation by transvaginal oocyte retrieval.Methods:The objects of the study were patients with polycystic ovary syndrome (PCOS) who underwent laparoscopic surgery in the Affiliated Reproductive Hospital of Shandong University from April to June 2020 and patients with tumors who underwent fertility preservation during gynecological surgery. This was a retrospective study. Totally 23 patients with PCOS infertility who were to undergo laparoscopy surgery underwent ovarian surface follicle puncture for oocyte retrieval, and the oocytes were obtained and sent to the assisted reproduction laboratory for in vitro culture, and mature oocytes were inseminated by intracytoplasmic sperm injection (ICSI), and high-quality embryos were obtained for cryopreservation. Gynecological surgery and ovarian surface follicle puncture were performed for oocytes retrieval in tumor patients ( n=6) with fertility preservation. The feasibility of simultaneous gynecological surgery with oocytes retrieved was evaluated according to the indexes of the number of oocytes retrieval, maturation, fertilization and embryo culture. Results:In PCOS patients, oocytes retrieved rate was 52.17% (12/23) per cycle. The number of oocytes retrieved was 2.00±1.48, among which the number of culturable oocytes was 1.50±1.00. The oocyte maturation rate was 22.22% (4/18). Two oocytes got fertilization and one zygote got cleavage. In contrast, the fertility-preserving oncology patients undergoing gynecologic surgery had a 100.00% (6/6) oocytes retrieved rate per cycle, with 3.00±1.27 oocytes retrieved. Oocyte maturation rate was 38.46% (5/13). Three oocytes got fertilization and one blastocyst formed. Three patients got oocytes or embryos cryopreservation.Conclusion:For patients who are not suitable for transvaginal oocyte retrieval for fertility preservation, follicle puncture during the gynecologic operation can yield available oocytes.
