Effect of quercetin on immune escape of gastric cancer cells by regulating PD-1/PD-L1 signaling pathway
10.3969/j.issn.1000-484X.2025.08.028
- VernacularTitle:槲皮素调节PD-1/PD-L1信号通路对胃癌细胞免疫逃逸的影响
- Author:
Yongjin HU
1
;
Feng'e LI
1
Author Information
1. 南开大学附属北辰医院肿瘤科,天津 300400
- Publication Type:Journal Article
- Keywords:
Gastric cancer;
Immune escape;
Quercetin;
Programmed death protein-1/programmed death ligand-1 signaling pathway
- From:
Chinese Journal of Immunology
2025;41(8):1965-1969
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of quercetin(QUE)on immune escape of gastric cancer cells by regulating the programmed death protein-1/programmed death ligand-1(PD-1/PD-L1)signaling pathway.Methods:Different concentrations of QUE(0～160 μmol/L)were applied to gastric cancer cell HGC-27,the cell survival rate was measured to screen for the optimal drug con-centration.HGC-27 cells were stochastically grouped into HGC-27 group,QUE low concentration(QUE-L)group,QUE medium con-centration(QUE-M)group,QUE high concentration(QUE-H)group,QUE-H+pcDNA-NC group(negative control for overexpres-sion of PD-1),and QUE-H+pcDNA-PD-1 group(overexpression of PD-1).Cell proliferation activity was analyzed by CCK-8 assay and colony formation assay.Apoptosis rate was detected by flow cytometry.The expression levels of proteins related to the PD-1/PD-L1 signaling pathway were detected by Western blot.Each group was co-cultured with NK cells,and the HGC-27 co-culture group,QUE-L co-culture group,QUE-M co-culture group,QUE-H co-culture group,QUE-H+pcDNA-NC co-culture group,and QUE-H+pcDNA-PD-1 co-culture group were constructed.NK cell killing rate was detected.ELISA was applied to detect the expression levels of IL-2,IFN-γ and TNF-α in various cell culture media.Results:Compared with the HGC-27 group,the cell survival rate,clone formation quantity,and PD-1 and PD-L1 expression levels in the QUE-L group,QUE-M group and QUE-H group were gradually decreased(P<0.05),while the cell apoptosis rate gradually increased(P<0.05).Moreover,the NK cell killing rate and the expression levels of IL-2,IFN-γ and TNF-α in the cell culture medium of the QUE-L co-culture group,QUE-M co-culture group,and QUE-H co-culture group gradually increased compared to the HGC-27 co-culture group(P<0.05).On the basis of high concentration QUE treatment of HGC-27 cells,overexpression of PD-1 reversed the trend of changes in the above indicators(P<0.05).Conclusion:QUE may inhibit immune escape of gastric cancer cells by down-regulating PD-1/PD-L1 signaling pathway.
