Angiotensin(1-7)alleviates apoptosis in β-cells under high-glucose and high-fat conditions by enhancing autophagy
10.3969/j.issn.1000-4718.2025.01.016
- VernacularTitle:Ang(1-7)对高糖高脂培养的胰岛β细胞自噬的影响
- Author:
Donghui HU
1
;
Mingjin SUN
;
Chunli LU
Author Information
1. 湖北医药学院附属随州医院生殖医学科,湖北 随州 441300
- Publication Type:Journal Article
- Keywords:
angiotensin(1-7);
pancreatic β cells;
glucolipotoxicity;
autophagy
- From:
Chinese Journal of Pathophysiology
2025;41(1):136-141
- CountryChina
- Language:Chinese
-
Abstract:
AIM:We aimed to evaluate the effect of angiotensin(Ang)(1-7)on autophagy in MIN6 cells un-der high-palmitate(PA)and high-glucose culture conditions.METHODS:MIN6 cells were cultured under various con-ditions:Dulbecco's Modified Eagle Medium(control,CON),high glucose and high PA concentrations(GLT),Ang(1-7)plus high glucose and high PA concentrations[Ang(1-7)+GLT],A779 inhibitor plus Ang(1-7)plus high glucose and high PA concentrations[A779+Ang(1-7)+GLT],and 3-methyladenine(3-MA)plus Ang(1-7)plus high glucose and high PA concentrations[3-MA+Ang(1-7)+GLT].The expression of microtubule-associated protein 1 light chain 3(LC3)was assessed using immunofluorescence,and Western blot was used to measure the expression of the autophagy-related proteins LC3-Ⅱ/LC3-Ⅰ and P62.Apoptosis was quantified using flow cytometry.RESULTS:GLT cells had fewer cytoplas-mic organelles,more vacuolated cells,an altered autophagosome distribution,higher P62 expression(P＜0.05),and greater apoptosis(P＜0.05)than CON cells.Ang(1-7)+GLT cells showed greater autophagosome formation,much less cy-toplasmic vacuolization,a higher LC3-Ⅱ/LC3-Ⅰ ratio(P＜0.05),lower P62 expression(P＜0.05),and less apoptosis(P＜0.05)than CON cells.A779 negated the pro-autophagic effects of Ang(1-7)in the MIN6 cells.Furthermore,inhibition of autophagy with 3-MA resulted in significantly greater apoptosis in 3-MA+Ang(1-7)+GLT cells vs Ang(1-7)-treated cells.CONCLUSION:Ang(1-7)activates Mas receptors to promote autophagy in pancreatic β cells cultured under high-glucose and high-fat conditions,thereby mitigating apoptosis and cellular pathology.
