Analysis of Anti-inflammatory Activities and Effective Components of Six Species of Selaginella
10.11842/wst.20231012001
- VernacularTitle:六种卷柏属药材的抗炎活性及有效成分分析
- Author:
You WU
1
;
Xueyan ZHANG
1
;
Zihan LI
1
;
Su XU
1
;
Keli CHEN
1
;
Juan LI
1
Author Information
1. 湖北中医药大学药学院 武汉 430065
- Publication Type:Journal Article
- Keywords:
Selaginella;
Anti-inflammatory activity;
RAW264.7 cells;
Lymphocytes;
Biflavonoid
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2025;27(1):143-153
- CountryChina
- Language:Chinese
-
Abstract:
Objective The anti-inflammatory activity of medicinal plants in the six species of Selaginella genus were compared,and their effective parts and components were analyzed.Methods The total flavonoid content of various extraction parts and the main components of ethyl acetate part of Selaginella were determined by ultraviolet and high-performance liquid chromatography(HPLC),respectively.In vitro inflammatory cell models were constructed using Concanavalin A(ConA)and lipopolysaccharide(LPS)induced rat splenic lymphocytes and mouse macrophages,and cell viability was measured by CCK-8 methods.The nitric oxide(NO)level was determined by the Griess method,and the levels of IL-1β and TNF-α were determined by ELISA.The mRNA expression of inducible nitric oxide synthase(iNOS)and TNF-α,among other inflammatory factors,were detected by RT-PCR.Results The flavonoid content of the ethyl acetate part of Selaginella was relatively high,and biflavonoids such as amentoflavone was the main components.All the extraction parts of the six species of Selaginella showed no significant cytotoxicity.The ethyl acetate part,roberstonin-4'-methylether,and amentoflavone could significantly reduce the release of NO in RAW264.7 cells or lymphocytes,and the ethyl acetate part of Selaginella labordei were the most effective in both models.Selaginella labordei had a rich variety of biflavonoid components,with roberstonin-type biflavonoids relatively higher than other species.The mechanism of action was related to the inhibition of inflammatory factors such as IL-1β and TNF-α levels,the downregulation of mRNA expression of TNF-α,IL-6,IL-1β,iNOS,and others.Conclusion All six species of Selaginella have certain in vitro anti-inflammatory effects and Selaginella labordei showed stronger activity,with biflavonoids as their main active components.
