Protective effect and mechanism of resuscitation solution combining adenosine,lidocaine and magnesium sulfate on endothelial cells in acute lung injury during sepsis
10.12007/j.issn.0258-4646.2025.08.002
- VernacularTitle:腺苷-利多卡因-硫酸镁复苏液对脓毒症急性肺损伤中内皮细胞的保护作用及机制
- Author:
Tingting LI
1
;
Jiong XIONG
;
Fuyu DENG
;
Xu LIU
;
Feng SHEN
;
Yan TANG
Author Information
1. 贵州医科大学临床医学院重症医学教研室,贵阳 550004
- Publication Type:Journal Article
- Keywords:
sepsis;
acute lung injury;
fluid resuscitation;
inflammatory cytokine
- From:
Journal of China Medical University
2025;54(8):678-683,689
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect and mechanism of adenosine-lidocaine-magnesium sulfate(ALM)resuscita-tion solution against acute lung injury in sepsis were investigated through in vitro and in vivo experiments.Methods ALM resuscitation solution effects on septic lung injury were assessed in SD rats and rat pulmonary micro vascular endothelial cells(PMVEC)in vitro,and the effector mechanism was explored by using network pharmacology combined with molecular biology methods,detecting alterations in nuclear factor kappaB(NF-κB)signaling pathway and inflammatory cytokine key protein expressions.Results Based on in vivo experi-ments,ALM resuscitation solution treatment significantly improved lung histopathological injury in cecal ligation and puncture(CLP)model rats,reduced the lung injury score and lung dry-to-wet ratio(P＜0.01),and significantly suppressed pro-inflammatory cytokine expressions such as that of IL-1β,IL-6,and TNF-α(P＜0.01).Moreover,in vitro experiments confirmed that ALM resuscitation solution significantly reduced p-p65 and p-IκBα protein expressions in LPS-induced PMVEC(P＜0.05),while down-regulating IL-1β,IL-6,and TNF-α protein levels(P＜0.01).Conclusion ALM resuscitation solution exerts a protective effect against acute lung injury in sepsis by inhibiting NF-κB signaling pathway activation and reducing pro-inflammatory cytokine release.
