Study on the relationship between the expression of GLUT8 and sperm motility and morphology in asthenozoospermia and teratozoospermia patients
10.3760/cma.j.cn101441-20210922-00422
- VernacularTitle:弱畸精子症患者精子中GLUT8的表达与精子活力和形态参数关系的研究
- Author:
Runfa GUO
1
;
Xuefang LI
;
Wenkai ZHU
;
Lixin ZHANG
;
Hongshan GE
Author Information
1. 南京医科大学附属泰州人民医院生殖医学科，泰州 225300
- Publication Type:Journal Article
- Keywords:
Sperm motility;
Teratozoospermia;
Glucose transporter 8
- From:
Chinese Journal of Reproduction and Contraception
2022;42(12):1259-1264
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the difference of facilitative glucose transporter 8 (GLUT8) expression in sperms among asthenozoospermia (AS) patients, teratozoospermia (TM) patients and normal reproductive men, thus to investigate the correlation between the expression of GLUT8 and sperm motility.Methods:The sperm motility parameters of each group were detected by computer-assisted semen analysis system (CASA). A case-control study method was used to collect semen from patients with AS, TM and normal reproductive men who underwent routine semen examination in the Andrology Laboratory, Department of Reproductive Medicine, the Affiliated Taizhou People's Hospital of Nanjing Medical University from December 2019 to December 2020. The semen of AS patients (41 samples), normal viable spermatozoa (NV) patients (41 samples), TM patients (40 samples) and normal morphology spermatozoa (NM) patients (40 samples) were collected and selected into AS group, NV group, TM group and NM group according to the criteria of enrollment. Western blotting was used to assess the expression of GLUT8 protein in NV group, AS group, NM group and TM group, with 7 cases in each group. The differences of sperm parameters and GLUT8 protein between NV group and AS group, NM group and TM group were compared.Results:Sperm parameters of NV group and AS group were significantly different in progressive rate (62.64%±37.14%, 14.41%±11.89%, P<0.001), motility rate (55.62%±12.31%, 24.40%±12.75%, P<0.001), curvilinear velocity [(87.22±12.35) μm/s, (71.75±18.35) μm/s, P<0.001], the straight line velocity [(40.22±6.71) μm/s, (33.31±10.11) μm/s, P<0.001], average path velocity [(47.66±6.49) μm/s, (39.42±10.25) μm/s, P<0.001], and amplitude of lateral head displacement [(2.88±0.49) μm, (2.35±0.66) μm, P<0.001], and there were no significant differences in sperm parameters between NM group and TM group (all P>0.05). The relative expression of GLUT8 protein in AS group (0.26±0.17) was significantly lower than that in NV group (2.00±0.38, P=0.002), but there was no significant difference in GLUT8 protein expression in NM group and TM group ( P>0.05). Conclusion:GLUT8 protein expression in sperm decreased in AS, but there was no significant correlation between GLUT8 protein expression and sperm morphology.
