Expression of GFP+cells and their response to diphtheria toxin in a Lyz2 IRES DTREGFP mice strain in vivo
10.3969/j.issn.1005-4847.2024.12.003
- VernacularTitle:一种Lyz2 IRES DTREGFP小鼠品系体内GFP+细胞的表达特征和对白喉毒素的反应特征
- Author:
Huazheng YAN
1
;
Jianxiong GAO
1
;
Nan ZHANG
1
;
Mingming BIAN
1
;
Yaomei XU
1
;
Lin ZHANG
1
;
Shiyu ZHOU
1
;
Yiwan FANG
1
;
Hezuo LYU
1
Author Information
1. 蚌埠医科大学第一附属医院检验科,安徽 蚌埠 233004;蚌埠医科大学慢性疾病免疫学基础与临床安徽省重点实验室,安徽 蚌埠 233030
- Publication Type:Journal Article
- Keywords:
transgenic mouse;
diphtheria toxin;
diphtheria toxin receptor;
depletion assay
- From:
Acta Laboratorium Animalis Scientia Sinica
2024;32(12):1524-1531
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression characteristics of green fluorescent protein(GFP)-positive myeloid cells(mainly monocytes,macrophages,and neutrophils)and their pattern of response to diphtheria toxin(DT)in Lyz2 internal ribosome entry site(IRES)DT receptor/enhanced GFP(DTREGFP)mice.Methods Transgenic Lyz2 IRES DTREGFP mice(6～8 weeks old)and C57BL/6J mice with the same genetic background were selected randomly and injected intraperitoneally with DT(25 ng/g)for 3 consecutive days.Bone marrow,peripheral blood,and spleen cells were separated and made into single-cell suspensions with GFP and CD11b as markers.Changes in the proportion of GFP+cells to total myeloid cells(CD11b+)was detected in these tissues at different time points before and after drug administration,using flow cytometry.Results Peripheral blood CD11b+GFP+cells accounted for(24.62±5.655)％of CD11b+cells before DT injection,and this proportion gradually decreased after injection and reached a minimum of(7.982±2.729)％on the third day.The proportions in spleen and bone marrow were(13.73±2.994)％and(65.23±4.261)％before DT injection,respectively,decreased most significantly on the first day after injection to(3.468±0.5862)％and(50.98±7.957)％,respectively,and then gradually recovered.Conclusions DT effectively reduced the proportions of monocytes,macrophages,and neutrophils in Lyz2 IRES DTREGFP mice,but the pattern of change varied among tissues.
