Antimicrobial Properties of Rare-earth Organic Acid Complexes (Re-SOA)
10.13865/j.cnki.cjbmb.2024.11.1218
- VernacularTitle:有机酸稀土配合物的抗菌性能
- Author:
Yu-Huan QIN
1
;
Hui QIN
;
Meng-Yang HUANG
;
Sheng-Hui PU
;
Wei LUO
;
Xue-Pin LIAO
;
Bi SHI
Author Information
1. 四川大学生物质科学与工程学院,成都610065
- Publication Type:Journal Article
- Keywords:
small-molecule organic acid (SOA);
rare earths (Re);
complexes;
poultry and livestock pathogens;
antimicrobial activity
- From:
Chinese Journal of Biochemistry and Molecular Biology
2024;40(12):1709-1722
- CountryChina
- Language:Chinese
-
Abstract:
The overuse of antibiotics in animal husbandry has resulted in escalating animal safety con-cerns,highlighting the urgent need for a safe and effective alternative to traditional acidifiers.necessita-ting the development of safe and effective alternatives to traditional acidifiers.This study investigates the antibacterial activity and underlying mechanisms of rare-earth organic acid complexes (Re-SOA),a promising alternative to traditional acidifiers,against three common poultry and livestock pathogens:Escherichia coli,Staphylococcus aureus,and Salmonella.A series of Re-SOA were synthesized via a liq-uid-phase method.Structural characterization using infrared spectroscopy,X-ray photoelectron spectros-copy,and Raman spectroscopy confirmed successful complexation.The minimum inhibitory concentration (MIC) and minimum sterilization concentration (MBC) were determined using the Oxford cup method and agar dilution method,respectively.The results indicated a synergistic antibacterial effect of the Re-SOA complexes,demonstrating significantly enhanced antibacterial activity compared to organic acids (SOA) and rare-earth ions (Re3+) alone.The antibacterial activity of the Re-SOA complexes followed the order:Yb-SOA>Gd-SOA>La-SOA,with Yb-Ac (Ac:acetic acid) exhibiting MIC values of 0.27,0.53,and 0.53 mmol·L-1,and MBC values of 0.53,0.53,and 0.53 mmol·L-1 against the three path-ogenic bacteria,respectively.Further investigation into the cellular mechanisms of action using field e-mission scanning electron microscopy (FESEM),transmission electron microscopy (TEM),and tran-scriptome sequencing revealed that Re-SOA effectively inhibits bacterial growth by targeting key metabolic pathways,including carbohydrate metabolism,energy metabolism,amino acid metabolism,and biofilm formation.Re-SOA,as a novel antibacterial agent,exhibits high efficacy and safety.This research pro-vides a promising approach for developing novel antibacterial agents and offers a potential solution to the critical issue of antibiotic resistance in animal husbandry.
