Kui Jie Kang regulates intestinal FXR and affects bile acid metabolism in treatment of ulcerative colitis in mice
- VernacularTitle:溃结康调控肠道法尼醇X受体影响胆汁酸代谢治疗小鼠溃疡性结肠炎
- Author:
Rong-yi XU
1
;
Xiao-si LI
;
Jian-guo MA
;
Xue-qing YANG
;
Hua-ning WANG
;
Yan QI
Author Information
- Publication Type:Journal Article
- Keywords: Kui Jie Kang; ulcerative colitis; bile acids; gut microbiota; FXR; CYP7A1
- From: Chinese Pharmacological Bulletin 2025;41(2):383-391
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the effects of Kui Jie Kang(KJK)on modulating the farnesoid X receptor(FXR)pathway in the gut microbiota and bile acid metabolism in mice with ulcerative colitis(UC).Methods Mice were subjected to DSS-induced UC and randomly as-signed to the control(CON),model(MOD),and two KJK-dosed groups(KJK.H at 12.8 g·kg-1,KJK.L at 3.2 g·kg-1).Mouse body weight was recorded,and disease activity index(DAI)was scored.The his-topathological changes in colonic tissue were observed via HE staining,and the number of goblet cells and mucosal layer repair were assessed using PAS and Al-cian blue staining.Bile acid content in feces was measured using LC-MS/MS,gut microbiota composition was analyzed by 16S rRNA gene sequencing,and the expression of FXR target genes and related proteins was detected by RT-qPCR and Western blot.Results KJK significantly ameliorated colonic shortening,de-creased disease activity index in UC mice,reduced his-topathological scores,increased the number of goblet cells and mucus secretion,altered the levels of primary and secondary bile acids,and increased the relative a-bundance of beneficial bacteria such as Lactobacillus.Additionally,it significantly upregulated the expression of FXR and FGF15 mRNA and protein in colonic tissue and downregulated the expression of hepatic CYP7A1 mRNA,and the correlation analysis in this study clearly revealed a significant correlation between bile acid me-tabolism disorders and gut microbiota imbalance in UC.Conclusion KJK activates the intestinal FXR-FGF15-CYP7A1 pathway,thereby regulating bile acid metabolism and restoring gut microbiota balance,which may be key to its improvement of UC.
