Mechanism of dioscin inhibiting apoptosis in HT22 cells after OGD/R
- VernacularTitle:薯蓣皂苷抑制氧糖剥夺/复氧HT22细胞凋亡的机制
- Author:
Zi-xin CHEN
1
;
Zhi-hui CHEN
1
;
Wen-chuan LUO
1
;
Feng-lin RAO
1
;
Mei HUANG
1
;
Ya-ping CHEN
1
;
Li-hong NAN
1
Author Information
- Publication Type:Journal Article
- Keywords: dioscin; OGD/R; HT22; apoptosis; PARP-1; AIF
- From: Chinese Pharmacological Bulletin 2025;41(2):277-283
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the neuroprotective effect of dioscin(DIO)on hippocampal neurons(HT22)after oxygen glucose deprivation/reoxygen-ation(OGD/R)and its possible mechanism.Methods HT22 cells were treated with 0,2.5,5,10,20,40,80,160,and 320 mg·L-1DIO for 24 h,and the cell proliferation rate was detected by CCK-8 method.The concentration that was non-toxic to HT 2 2 cells was se-lected for subsequent experiments.After OGD for 2 h,HT22 cells were randomly divided into the OGD/R group,1.25,2.5,and 5 mg·L-1DIO group,and posi-tive control group.HT22 cells were taken as the con-trol group.After drug intervention for 24 h,the cell proliferation rate was detected by CCK-8 method;the LDH release was detected by colorimetry;the cell ap-optosis rate was detected by TUNEL method;the ex-pression of proteins related to PARP-1/AIF pathway and caspase pathway was detected by Western blot.Results DIO intervention significantly upregulated the expression of AIF protein in mitochondria and PAR protein in nucleus of HT22 cells after OGD/R,and sig-nificantly downregulated the release of LDH,neuronal apoptosis rate,total protein expression of AIF and PAR,PARP-1,AIF in nucleus and protein expression of PAR protein in mitochondria,while the expression of Bax and caspase-3 proteins was not significantly differ-ent from that in the OGD/R group.Conclusion DIO can alleviate the apoptosis of HT22 cells induced by OGD/R by regulating the expression and translocation of proteins related to the PARP-1/AIF pathway,thus playing a neuroprotective role.
