Scutellarin alleviates cerebral ischemia-reperfusion injury in rats through CX3CL1/CX3CR1-PI3K/AKT pathway
10.16557/j.cnki.1000-7547.2025.04.008
- VernacularTitle:灯盏花乙素通过CX3CL1/CX3CR1-PI3K/AKT通路减轻大鼠脑缺血再灌注损伤
- Author:
Jun LI
1
;
Yi HOU
;
Ronghui LIU
;
Wenchao HU
;
Song TAO
;
Hong HUANG
Author Information
1. 昆明医科大学第二附属医院,昆明 650106
- Publication Type:Journal Article
- Keywords:
cerebral ischemia-reperfusion injury(CIRI);
scutellarin(Scu);
PI3K/AKT signaling pathway;
CX3CL1/CX3CR1 signaling pathway;
rat
- From:
Chinese Journal of Neuroanatomy
2025;41(4):469-476
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the neuroprotective efficacy of scutellarin(Scu)against cerebral ischemia-reperfusion(CIRI)injury in rats,with a focus on its regulatory mechanisms involving the CX3CL1/CX3CR1-PI3K/AKT signaling axis.Methods:The rat CIRI model was established using Longa's intraluminal suture method.The experimental design comprised four groups(n=15 per group):Sham operation group,CIRI model group,Scu treat-ment(40 mg/kg/d Scu gavage)group,and Scu+LY294002(Scu combined with PI3K inhibitor LY294002)group.Following 4 weeks of intervention,neurological function,pathological changes,and pathway protein expression were evaluated using Longa scoring,TTC staining,HE staining,transmission electron microscopy,immunofluorescence,or Western blot analysis.Results:Compared to the CIRI model group,Scu treatment significantly improved neurological function,reduced cerebral infarct volume and attenuated neuronal damage(P<0.05).Furthermore,Scu downregulat-ed CX3CL1/CX3CR1 expression,increased p-PI3K/PI3K and p-AKT/AKT ratios,elevated Bcl-2/Bax ratio,and de-creased IL-17A expression(P<0.05).These protective effects were partially reversed by LY294002.Conclusion:Scu exerts neuroprotective effects in cerebral CIRI injury by downregulating CX3CL1/CX3CR1 expression and activating the PI3K/AKT pathway,thereby attenuating inflammation and apoptosis.
