Whole-genome sequencing and colistin resistance of carbapenem-resistant Klebsiella pneumoniae
10.12138/j.issn.1671-9638.20256485
- VernacularTitle:耐碳青霉烯类肺炎克雷伯菌全基因组测序及黏菌素耐药分析
- Author:
Wenwen CHU
1
;
Zhou LIU
1
;
Xin LI
1
;
Naifang YE
1
;
Zhen GONG
1
;
Xiaojiao ZENG
1
;
Qiang ZHOU
1
Author Information
1. 安徽医科大学第二附属医院检验科,安徽 合肥 230601
- Publication Type:Journal Article
- Keywords:
carbapenem-resistant Klebsiella pneumoniae;
whole-genome sequencing;
single nucleotide polymor-phism analysis;
colistin;
antimicrobial resistance;
ISKpn26
- From:
Chinese Journal of Infection Control
2025;24(1):37-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the molecular epidemiology and colistin-resistant genes of carbapenem-resistant Klebsiella pneumoniae(CRKP)by whole-genome sequencing,and to provide reference for clinical diagnosis and treatment.Methods 57 CRKP strains isolated from clinical specimens of hospitalized patients in a tertiary general first-class hospital in Anhui Province from 2021 to 2023 were collected and antimicrobial susceptibility testing was performed.Multilocus sequence typing,capsule serotype,resistance genes,and virulence genes of CRKP strains were analyzed by whole-genome sequencing technique,and single nucleotide polymorphism analysis was conducted on sequences of all strains.Colistin resistance-related genes were amplified by polymerase chain reaction(PCR).Results 57 CRKP strains exhibited resistance to 14 antimicrobial agents,with the exception of tigecycline.The se-quencing results showed that 93.0％(53/57)of CRKP carried blaKPC-2,and the ST11 type CRKP strain had the highest detection rate(51/57,89.5％).Single nucleotide polymorphism clustering analysis showed that the 57 CRKP strains were divided into 11 clone groups,of which 4 clone groups were all ST11-KL64 type CRKP.40(70.2％)CRKP strains carried multiple virulence genes.Five strains of CRKP were colistin-resistant strains,the resistance mechanism involved the insertion of ISKpn26 element at site 70 of the mgrB gene.Conclusion The CRKP strain is primarily characterized by the production of KPC-2 ST11-KL64,with disseminated transmission in intensive care unit.The insertion of ISKpn26 element leading to mgrB gene mutation is related to resistance of CRKP to colistin in this region.
