The effects of acupuncture on the expression of circRNA_017109 and miR-138-5p in rat hippocampus post ischemia-reperfusion injury
10.16557/j.cnki.1000-7547.2025.04.005
- VernacularTitle:针刺对脑缺血再灌注损伤大鼠海马circRNA_017109和miR-138-5p表达的影响
- Author:
Litong CHEN
1
;
Canming XIE
;
Yao WANG
;
Jia YUAN
;
Xiaoyue LIU
;
Haomei TIAN
;
Chutao CHEN
;
Kun AI
Author Information
1. 湖南中医药大学针灸推拿与康复学院,长沙 410208
- Publication Type:Journal Article
- Keywords:
acupuncture;
cerebral ischemia-reperfusion injury(CIRI);
hippocampus;
circRNA_017109;
miR-138-5p;
inflammation;
rat
- From:
Chinese Journal of Neuroanatomy
2025;41(4):445-451
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of acupuncture intervention on the expression of circRNA_017109 and miR-138-5p in the hippocampal region of rats with cerebral ischemia-reperfusion injury(CIRI),and to explore the potential mechanism of acupuncture in treating CIRI.Methods:Forty-five Sprague-Dawley(SD)rats were divided into sham group,middle cerebral artery occlusion/reperfusion(MCAO/R)group,and acupuncture(MCAO/R+AC)group.The MCAO/R and MCAO/R+AC groups were subjected to the modified Longa suture method to establish the MCAO/R model.The MCAO/R+AC group received acupuncture at the"Dazhui","Renzhong",and"Baihui"acu-points.Neurological impairment was assessed via the modified neurological severity score(mNSS).while cerebral infarct volume was assessed through TTC staining.Histological alterations in brain tissue were examined via HE stai-ning,and the expression of circRNA_017109 and miR-138-5p in the hippocampal area were determined using RT-qPCR.Results:The mNSS scores and cerebral infarct volume ratios in the model groups were markedly higher than those in the sham group(P<0.01),but these were significantly reduced in MCAO/R+AC group compared to the MCAO/R group(P<0.01).HE staining showed severe necrosis and inflammation in the MCAO/R group's hippocam-pus,while the MCAO/R+AC group had minimal necrosis and scattered inflammation.RT-qPCR results indicated that the expression of circRNA_017109 and miR-138-5p was downregulated in the MCAO/R group,while MCAO/R+AC group upregulated the expression of circRNA_017109 and miR-138-5p(P<0.05,P<0.01).Conclusion:Acupunc-ture can improve neurological deficits,reduce cerebral infarction volume,and alleviate inflammatory cell infiltration in CIRI rats by upregulating the expression of circRNA_017109 and miR-138-5p,thereby exerting neuroprotective effects.
