The glutamatergic projections from basal forebrain to lateral habenula mediate the regulation of anesthesia in mice
10.16557/j.cnki.1000-7547.2025.04.004
- VernacularTitle:小鼠基底前脑谷氨酸能神经元至外侧缰核的投射介导麻醉调节
- Author:
Shiyi ZHAO
1
;
Ze FAN
;
Hailong DONG
Author Information
1. 空军军医大学:西京医院麻醉与围术期医学科,西安 710032
- Publication Type:Journal Article
- Keywords:
basal forebrain(BF);
glutamate;
isoflurane;
lateral habenula(LHb);
optogenetic;
mouse
- From:
Chinese Journal of Neuroanatomy
2025;41(4):437-444
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the efferent projections of glutamatergic neurons in the basal forebrain(BF)and the function of its projections to lateral habenula(LHb)under isoflurane anesthesia.Methods:Channelrhodopsin-as-sisted circuit mapping(CRACM)was used to explore the efferent projections of BF glutamatergic neurons in cre driver mice lines.On this basis,we optogenetically activated the BF glutamatergic projections to LHb to explore its function in wake-anesthesia regulation of isoflurane anesthesia.Results:The glutamatergic projections of BF mainly project to LHb,optogenetic activation of BF glutamatergic projections to LHb could significantly prolonged the time of loss of righ-ting reflex(LORR)and shortened the time of recovery of righting reflex(RORR).Optical stimulation of this projection reduced the burst suppression ratio(BSR)under 1.0%isoflurane anesthesia.When the mice were anesthetized by 0.8%isoflurane,a significant decrease of δ power percentage was observed,while the power percentage of β bands and γ bands increased.Conclusion:The glutamatergic neurons of BF mainly project to LHb,activation of this projection can not only prolong the induction time but also shorten the emergence time.Furthermore,this projection also exerts a pro-arousal effect under the maintenance phase of isoflurane anesthesia.
