Salidroside attenuates inflammatory response in mice with polycystic ovary syndrome by inhibiting NF-κB signaling pathway
10.3760/cma.j.cn101441-20201126-00646
- VernacularTitle:红景天苷通过抑制NF-κB通路降低多囊卵巢综合征模型小鼠的炎症反应
- Author:
Rui JI
1
;
Yue GAO
1
;
Wen ZOU
1
;
Jing YANG
1
Author Information
1. 武汉大学人民医院生殖医学中心，湖北省辅助生殖与胚胎发育医学临床研究中心，武汉430060
- Publication Type:Journal Article
- Keywords:
Polycystic ovary syndrome;
Granulosa cells;
Nuclear factor-κB;
Infammation;
Salidroside
- From:
Chinese Journal of Reproduction and Contraception
2022;42(6):595-603
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the regulatory effects of salidroside (Sal) on mice in an inflammatory state with polycystic ovary syndrome (PCOS) and the link with nuclear factor-κB (NF-κB) signaling pathway.Methods:Totally twenty-eight female C57B/6J mice aged 21 d were divided into control group, Sal group, PCOS group and PCOS+Sal group with 7 mice per group based on numbers randomly generated by computer and 21 d treatment was taken. Glucose tolerance test (GTT) was performed when the treatment was finished, ovarian tissues were taken for HE staining and morphological analysis, immunohistochemistry was used to detect tumor necrosis factor (TNF)-α and p-NF-κB p65 protein levels in the ovaries, and enzyme-linked immunsorbent assay (ELISA) was taken to detect the levels of serum sex hormones and inflammatory cytokines. Human ovarian granulosa cell line (KGN cells) were divided into four groups: control group, Sal (30 μmol/L) group, lipopolysaccharide (LPS) (0.5 mg/L) group and LPS (0.5 mg/L) +Sal (15 μmol/L, 30 μmol/L, 60 μmol/L) group. Inflammatory cytokines mRNA levels and nuclear factor (NF)-κB associated protein levels were detected after culture for 24 h.Results:The levels of testosterone [(2.11±0.60) μg/L vs. (0.77±0.06) μg/L, P<0.001], luteinizing hormone (LH) [(2.54±0.27) U/L vs. (1.07±0.21) U/L, P<0.001], LH/follicle-stimulating hormone (FSH) (0.12±0.01 vs. 0.05±0.01, P<0.001) and inflammatory cytokines interleukin (IL)-1β [(107.83±12.05) ng/L vs. (33.83±4.96) ng/L, P<0.001], IL-6 [(56.70±7.33) ng/L vs. (28.91±8.53) ng/L, P<0.001], TNF-α [(74.72±14.64) ng/L vs. (37.07±5.48) ng/L, P<0.001] increased in PCOS mice compared with control group, but the levels of testosterone [(1.47±0.25) μg/L, P=0.012], LH [(1.73±0.17) U/L, P<0.001], LH/FSH (0.08±0.01, P<0.001), IL-1β [(74.21±10.64) ng/L, P<0.001], IL-6 [(40.90±5.01) ng/L, P=0.002] and TNF-α [(55.42±8.78) ng/L, P=0.017] decreased significantly following Sal treatment. Immunohistochemical staining showed that TNF-α and p-NF-κB p65 protein levels in granulosa cells increased in PCOS mice but decreased after Sal treatment. Besides, IL-1β (1.76±0.09 vs. 0.50±0.20, P<0.001), IL-6 (1.79±0.20 vs. 0.80±0.10, P<0.001), TNF-α (1.56±0.19 vs. 0.70±0.30, P<0.001) mRNA levels increased significantly in LPS induced KGN cells, after Sal treatment (15 μmol/L, 30 μmol/L and 60 μmol/L), the mRNA levels of IL-1β (1.09±0.07, 1.00±0.02, 0.96±0.03, all P<0.001), IL-6 (1.47±0.12, 0.93±0.18, 0.91±0.27, P<0.001) and TNF-α (1.21±0.25, 0.94±0.35, 0.76±0.07, all P<0.001) apparently decreased. In addition, p-NF-κB p65 (0.49±0.07 vs. 0.31±0.03, P=0.013) and p-IκBα (0.48±0.06 vs. 0.26±0.04, P=0.012) protein levels increased apparently in LPS induced KGN cells, but the protein levels of p-NF-κB p65 (0.33±0.05, P=0.024) and p-IκBα (0.31±0.06, P=0.046) decreased following Sal treatment. Conclusion:Sal can attenuate inflammation levels in mice with PCOS by inhibiting NF-κB signaling pathway.
