Melatonin promotes mitophagy in rats with subarachnoid hemorrhage through PINK1/Parkin pathway
10.16557/j.cnki.1000-7547.2025.04.013
- VernacularTitle:褪黑素通过PINK1/Parkin通路促进蛛网膜下腔出血大鼠线粒体自噬
- Author:
Weilong SONG
1
;
Guangzhi HAO
1
;
Yong LIANG
1
;
Yushu DONG
1
Author Information
1. 中国人民解放军北部战区总医院神经外科,沈阳 110016
- Publication Type:Journal Article
- Keywords:
melatonin;
PINK1/Parkin;
mitophagy;
subarachnoid hemorrhage(SAH);
early brain injury(EBI);
rat
- From:
Chinese Journal of Neuroanatomy
2025;41(4):507-511
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of melatonin on mitophagy in the brain tissue of rats with early subarach-noid hemorrhage(SAH).Methods:The rat model of SAH was established by internal carotid artery puncture and trea-ted with melatonin by intraperitoneal injection.At 24 hours after the operation,the neurological function of rats was de-tected by mNSS score and balance beam test.The contents of reactive oxygen species(ROS),malondialdehyde(MDA),and glutathione(GSH)and in brain tissue were detected by commercial reagent kits.The expression changes of PINK1/Parkin,microtubule-associated protein1A/1B-light chain3(LC3)and p62 in brain tissue were detected by Western blot.Results:Melatonin can significantly improve the neurological function of SAH rats,reduce the con-tents of MDA(P<0.05)and ROS(P<0.05),and increase the content of GSH(P<0.05).Meanwhile,Western blot detection indicated that melatonin could increase the expression of PINK1 and Parkin(P<0.05),increase the ratio of LC3-II/LC3-I(P<0.05),and reduce the expression of p62(P<0.05).Conclusion:Melatonin alleviates early brain injury in rats with subarachnoid hemorrhage through PINK1/Parkin-mediated mitophagy.
