miR-21 promotes the repair of sciatic nerve injury in rats by regulating the differentiation of bone marrow stromal cells
10.16557/j.cnki.1000-7547.2025.04.009
- VernacularTitle:miR-21通过调节骨髓基质细胞分化促进大鼠坐骨神经的损伤修复
- Author:
Caihong WEI
1
;
Yumei LIU
1
;
Heying WANG
1
;
Junping LI
1
;
Hua JIA
1
Author Information
1. 宁夏医科大学基础医学院人体解剖与组织胚胎学系,银川 750004
- Publication Type:Journal Article
- Keywords:
miR-21;
bone marrow stromal cells(BMSCs);
peripheral nerve injury(PNI);
Schwann cell-like cells(SCLCs);
differentiation;
rat
- From:
Chinese Journal of Neuroanatomy
2025;41(4):477-485
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the repair mechanism of bone marrow stromal cells(BMSCs)transfected with miR-21 in rat sciatic nerve injuries.Methods:In vitro,rat BMSCs were transfected with miR-21 agonist(agomir-21)or blocker(antagomir-21)and their respective empty vectors,respectively,and divided into Control group,agomir-21 group,agomir-NC group,antagomir-21 group and antagomir-NC group.A 12 mm rat sciatic nerve injury model contai-ning decellularized nerve area was prepared,and the cells in each group were labeled with Hoechst33342 for postopera-tive implantation of decellularized nerve area.At 14 d after operation,the recovery of neuromotor function was evaluated by footprint test and the wet weight ratio of gastrocnemius.Immunofluorescence staining was applied to detect the expression of neurofilament protein 200(NF200),myelin basic protein(MBP),S100 calcium binding protein β(S100β)and glial fibrillary acidic protein(GFAP)within the regenerating nerve.Results:Compared with Control group,the sciatic nerve function index(SFI)and the wet weight ratio of gastrocnemius were significantly increased in agomir-21 group(P<0.05);and the density of regenerated nerve fibers and the expression of myelin marker proteins were increased,and the differentiation rate of BMSCs into Schwann cell-like cells(SCLCs)was elevated significantly(P<0.05).Conclusion:miR-21 significantly promotes peripheral nerve regeneration by enhancing the differentiation of BMSCs into SCLCs in vivo.
