Molecular epidemiological characteristics and hypervirulence evolution of ST11 carbapenem-resistant Klebsiella pneumoniae in medical institutions in Shanghai
10.12138/j.issn.1671-9638.20257205
- VernacularTitle:上海市医疗机构ST11型耐碳青霉烯类肺炎克雷伯菌分子流行病学特征与高毒力进化研究
- Author:
Jing BI
1
;
Wenjie CHEN
;
Liang TIAN
;
Qian LIU
;
Huanyu WU
;
Min CHEN
;
Taiyao CHEN
;
Tingting SHI
;
Wei MA
;
Hongzhi ZHANG
Author Information
1. 上海市疾病预防控制中心病原生物检定所,上海 200336
- Publication Type:Journal Article
- Keywords:
Klebsiella pneumoniae;
serotyping;
MLST;
ST11 type;
hypervirulent carbapenem-resistant Kleb-siella pneumoniae;
hv-CRKP;
drug resistance gene;
virulence gene
- From:
Chinese Journal of Infection Control
2025;24(8):1075-1082
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the molecular epidemiological characteristics and hypervirulence evolution trend of ST11 carbapenem-resistant Klebsiella pneumoniae(CRKP)isolated from medical institutions in Shanghai,and provide scientific basis for formulating the prevention and control measures of drug-resistant organisms.Methods A total of 201 strains of CRKP isolated from 12 medical institutions in Shanghai from 2021 to 2022 were collected.Antimicrobial susceptibility testing and whole genome sequencing were performed.The concatenated data was used for multilocus sequencing typing(MLST),serum typing(wzi typing),as well as analysis of resistance and viru-lence genes.Results All 201 CRKP strains were multidrug-resistant organisms(MDROs).These strains were al-most completely resistant to carbapenems,cephalosporins,and quinolones.Drug resistance gene analysis showed that 93.03％of CRKP strains carried KPC gene.201 CRKP strains were divided into 6 ST types and 10 capsule wzi types,with ST11-KL64(n=104)being the dominant type,followed by ST15-KL19(n=54).52.24％(n=105)of CRKP carried rpmA/rpmA2+iucA+iutA+iroN genes.Conclusion CRKP isolated from medical institutions in Shanghai is mainly ST11-KL64 type with severe multidrug resistance,and more than half of the strains are hyper-virulent carbapenem-resistant Klebsiella pneumoniae(hv-CRKP).It is necessary to continuously strengthen the monitoring of the molecular characteristics of CRKP,so as to prevent outbreaks of healthcare-associated infection.
