Effect of medium incubated with low oxygen and high oxygen one day in advance on the development of embryos cultured and pregnancy outcomes in different insemination ways
10.3760/cma.j.cn101441-20210927-00437
- VernacularTitle:培养液提前一日低氧平衡和高氧平衡对不同授精方式胚胎早期发育及妊娠结局的影响
- Author:
Linlin TAO
1
;
Guozhen LI
1
;
Zhiwei YANG
1
;
Yasong GENG
1
;
Fangfang DAI
1
;
Bo ZHENG
1
Author Information
1. 邢台不孕不育专科医院生殖医学中心，邢台 054000
- Publication Type:Journal Article
- Keywords:
Fertilization in vitro;
Sperm injection, intracytoplasmic;
Developmental potential, embryo;
Pregnancy outcomes;
Low oxygen balance;
Medium
- From:
Chinese Journal of Reproduction and Contraception
2022;42(12):1234-1240
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the development of embryos cultured and pregnancy outcomes after in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) under medium incubated with low oxygen (5% oxygen concentration) and high oxygen (20% oxygen concentration) one day in advance. Methods:We retrospectively analyzed the data of 881 IVF/ICSI cycles from August 2018 to September 2019 in the Reproductive Medicine Center of Xingtai Infertility Specialist Hospital. The culture medium was prepared one day in advance and placed in an incubator for balance. According to the oxygen concentration in the incubator, it was divided into low oxygen balance group (5% oxygen concentration) and high oxygen balance group (20% oxygen concentration). The effects of two different medium balanced environments on embryonic development and pregnancy outcomes under two insemination methods were compared.Results:The number of oocytes retrieved, the fertilization rate, the two pronucleus (2PN) cleavage rate and the vailable embryo rate were not significantly different between the two groups of IVF and ICSI (all P>0.05). There was no significant difference on day 3 (D3) available embryo rate between the two groups of IVF, but it was significantly higher in the low oxygen balance group than in the high oxygen balance group of ICSI [68.52% (664/969) vs. 64.42% (795/1234), P=0.043]. The available blastocyst rate of the low oxygen balance group were significantly higher than that in the high oxygen balance group in IVF and ICSI [IVF: 44.99% (808/1796) vs. 41.45% (841/2029), P=0.027; ICSI: 42.00% (307/731) vs. 34.95% (317/907), P=0.004]. The number of transferred embryos in the low oxygen balance group was significantly lower than that in the high oxygen balance group in IVF and ICSI (IVF: 1.87±0.34 vs. 1.96±0.21, P=0.001; ICSI: 1.79±0.41 vs. 1.94±0.23, P=0.002). The implantation rate, the clinical pregnancy rate, and the live birth rate were not significantly different between the two groups of IVF and ICSI. Logistic regression showed that liquid balance environment was related to live birth rate ( OR=0.238, 95% CI=0.139-0.409, P<0.001). Conclusion:Compared with the high oxygen, medium incubated in the low oxygen (5% O 2 concentration) can enhance the developmental potential of embryos, significantly enhance live birth rate, and ICSI is more effective than IVF. But the exact results still need to be confirmed by a large sample of randomized controlled studies.
