Effects of 4-week moderate intensity treadmill exercise on pathological characteristics and Cytl1-CCR2 pathway activity of knee cartilage in MIA-induced knee osteoarthritis rats
10.3969/j.issn.1001-1242.2025.08.002
- VernacularTitle:4周中等强度跑台运动对碘乙酸钠诱导的膝骨关节炎大鼠软骨病理特征及Cytl1-CCR2通路活性的影响
- Author:
Tao JIANG
1
;
Jianing MIAO
;
Zheng WANG
Author Information
1. 沈阳体育学院运动健康学院,辽宁省沈阳市,110102
- Publication Type:Journal Article
- Keywords:
knee osteoarthritis;
treadmill exercise;
Cytl1-CCR2 pathway;
pathological characteristics of cartilage
- From:
Chinese Journal of Rehabilitation Medicine
2025;40(8):1137-1147
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of 4-week moderate intensity treadmil1 exercise on knee cartilage patholo-gy and the activity of Cytokine-like 1(Cytl1)-C-C motif chemokine receptor 2(CCR2)pathway in knee carti-lage of rats with monoiodoacetate(MIA)induced knee osteoarthritis(KOA).Method:Sixty SD rats were randomly divided into control group,control and exercise group,knee osteoarthritis group and knee osteoarthritis and exercise group.The KOA model was induced by intraarticular injection of MIA,and the moderate intensity treadmill exercise was carried out for 4 weeks after MIA injection.The changes of body weight and knee joint swelling were recorded dynamically;the osteophytes and deformities of knee joint were observed by X-ray and stereomicroscope;the structural changes of knee cartilage were evaluated by toluidine blue staining;the apoptosis of chondrocytes was analyzed by terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL);and the bioinformatics analysis of gene expression omnibus(GEO)data set was used to predict the potential key pathways of exercise alleviating KOA pathology.Immunohistochemical staining or Western Blot was used to detect the protein expression of related molecules in cartilage tissue.Result:The KOA rat model was successfully established,and 4-week moderate intensity treadmill exercise could alleviate the pathological characteristics of KOA and inhibit cartilage degeneration and apoptosis;bioinfor-matics analysis of GEO data set showed that Cytl1-CCR2 pathway might be involved in the alleviation of KOA by exercise;4-week moderate intensity treadmill exercise promoted the expression of Cytl1,while inhibit-ed the expression of proteins related to CCR2 pathway in cartilage tissue of KOA rats.Conclusion:4-week moderate intensity treadmill can improve the pathological characteristics of knee cartilage injury in KOA rats,which might be related to the activity of Cytl1-CCR2 pathway.
