Cyclosporine A improves mouse blastocyst implantation with mild impaired trophoblast cells
10.3760/cma.j.cn101441-20210114-00023
- VernacularTitle:环孢素A改善滋养细胞轻度受损小鼠囊胚的着床
- Author:
Menglan WU
1
;
Taomei LI
;
Jilong MAO
;
Yanlin MA
;
Qi LI
;
Yuanhua HUANG
Author Information
1. 郑州大学第一附属医院生殖医学中心，郑州 450000
- Publication Type:Journal Article
- Keywords:
Cyclosporine A;
Embryo implantation;
Trophoblast
- From:
Chinese Journal of Reproduction and Contraception
2022;42(7):711-716
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether the cyclosporin A (CsA) can improve the blastocyst implantation with mild impaired of trophoblast cells of mice.Methods:1) Totally 30 ICR mice were intraperitoneally injected with 5 mg/kg CsA, then the blood concentrations of CsA in mice were detected at 1 h, 3 h, 4 h, 6 h, 8 h, 10 h, 13 h, 14 h, 16 h and 20 h after administration using chemiluminescence method. 2) A total of 36 mice were randomly divided into control group and experimental group (CsA group); the experimental group was intraperitoneally injected with 5 mg/kg CsA and control group was injected with the equivalent dose olive oil of the same body weight. Embryos were classified into A, B, C types according to the guidelines of Gardner and experimental needs. Embryos were transferred to the uterus in the two groups. The embryo implantation rate was calculated and the leukemia inhibitory factor (LIF) mRNA level was measured by real time fluorescence quantitative PCR in 5.5 d postcoitum (dpc).Results:1) The blood concentration of CsA in mice reached a peak at 6-10 h after administration. 2) The embryo implantation rate of B type [73.9% (34/46)] had significantly improved in experimental group [50.0% (23/46), P=0.018]. 3) The expression of LIF mRNA level was not significantly different between the two groups ( P>0.05). Conclusion:When CsA was administered at a dose of 5 mg/kg, CsA can improve the embryo implantation rate with mild impaired trophoblast cells. CsA might become a potential drug to increase the success rates of the in vitro fertilization.
