The anti-heart failure mechanism of N-acetylcysteine in diabetic cardiomyopathy via ERK1/2 path-way
10.3969/j.issn.1008-0074.2025.04.20
- VernacularTitle:N—乙酰半胱氨酸在糖尿病性心肌病中经ERK1/2通路的抗心衰机制研究
- Author:
Jian JI
1
;
Ya-hong HUANG
1
;
Ying-min LU
1
;
Dong-mei YUE
1
;
Xiao-hui ZHENG
1
;
Jin-chun ZHANG
1
;
Zhao-xia WANG
1
Author Information
1. 上海健康医学院附属崇明医院心内科,上海 202150
- Publication Type:Journal Article
- Keywords:
Cardiomyopathies;
Diabetes mellitus;
Acetylcysteine;
Heart failure
- From:
Chinese Journal of cardiovascular Rehabilitation Medicine
2025;34(4):543-547
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the anti-heart failure mechanism of N-acetylcysteine(NAC)in diabetic cardiomyop-athy independent from coronary artery factors.Methods:A total of 40 diabetic mice after heart failure model construction were randomly divided into two groups,NAC group(n=20,NAC 100mg·kg-1·d-1)and control group(n=20,Saline 100 mg·kg-1·d-1).Echocardiography was performed to detect left ventricular end-diastolic volume(LVEDV),left ventricular end-systolic volume(LVESV),left ventricular ejection fraction(LVEF),mitral left ventricular early-dias-tolic peak flow velocity/left ventricular late-diastolic peak flow velocity(E/A),isovolumic relaxation time(IVRT)and cardiac output(CO)after 4 weeks.Terminal uridine nick-end labeling(TUNEL)was performed to detect apoptosis in-dex,and Western Blot was performed to detect the expression of extracellular regulated protein kinases(ERK)1/2 after 6 weeks in two groups.Results:Compared to those in control group,mice in NAC group had significant higher LVEF[(40.5±3.4)％vs.(36.9±3.2)％],E/A[(1.5±0.1)vs.(1.4±0.1)]and CO[(10.3±0.6)ml/min vs.(9.9±0.5)ml/min](P＜0.05 or＜0.01);and significant lower LVESV[(23.1±1.3)μl vs.(24.7±1.5)μl],apoptosis index[(31.2±0.5)％vs.(45.1±0.9)％]and the expression of ERK1/2[(2.2±0.2)vs.(3.9±0.1)](P＜0.001 all).Conclusion:NAC exerts anti-heart failure effect by attenuating apoptosis of cardiomyocytes via regulating ERK1/2 pathway.
