Correlation between FoxO1 mRNA, Caspase-3 mRNA, GDF-9 levels and the number of high-quality embryos and the efficacy of in vitro fertilization-embryo transfer in predicting clinical pregnancy in infertile patients
10.3760/cma.j.cn101441-20200925-00533
- VernacularTitle:卵泡液 FoxO1 mRNA、 Caspase-3 mRNA、GDF-9水平与优质胚胎数的相关性及其预测不孕患者IVF-ET临床妊娠的效能研究
- Author:
Xueying ZHANG
1
;
Haiying CAO
;
Tieqi HOU
;
Xiuling LIU
Author Information
1. 驻马店市中心医院妇科，驻马店 463000
- Publication Type:Journal Article
- Keywords:
Fertilization in vitro;
Embryo transfer;
Number of high-quality embryos;
Follicle-stimulating hormone;
Family O subgroup of fork-head frame transcri
- From:
Chinese Journal of Reproduction and Contraception
2022;42(4):364-371
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the correlation between Forkhead box transcription factor O1 (FoxO1) mRNA, Caspase-3 mRNA, growth differentiation factor-9 (GDF-9) expression levels and the number of high-quality embryos and the efficacy of predicting in vitro fertilization-embryo transfer (IVF-ET) clinical pregnancy in infertile patients. Methods:A case-control study of 468 infertile patients who received IVF-ET treatment from February 2017 to January 2020 in Zhumadian Central Hospital was performed. According to whether pregnant, patients were divided into pregnancy group ( n=248) and non-pregnancy group ( n=220). We compared the general data, sex hormone levels on the day of egg retrieval, FoxO1 mRNA, caspase-3 mRNA, and GDF-9 levels in follicular fluid between the two groups. Pearson was used to analyze the relationship between FoxO1 mRNA, Caspase-3 mRNA, and GDF-9 in follicular fluid and the number of high-quality embryos. The logistic regression equation was used to analyze the related factors of IVF-ET clinical pregnancy. The receiver operating characteristic (ROC) curve was used to analyzed the efficacy of follicular fluid FoxO1 mRNA, Caspase-3 mRNA, GDF-9 in predicting clinical pregnancy. Results:The number of high-quality embryos in pregnancy group was higher than that in non-pregnancy group (2.59±0.68 vs. 1.78±0.62, P<0.001), the basal follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were lower than those in non-pregnancy group [(7.16±0.29) U/L vs. (6.21±0.34) U/L, (8.72±1.24) U/L vs. (10.65±1.01) U/L, all P<0.001] and the estradiol on the day of oocytes retrieved was higher than that of non-pregnancy group [(848.18±50.68) ng/L vs. (605.35±46.79) ng/L, P<0.001]. FoxO1 mRNA and GDF-9 in pregnancy group were higher than those in non-pregnancy group, Caspase-3 mRNA was lower than that in non-pregnancy group ( P<0.001). FoxO1 mRNA was negatively correlated with Caspase-3 mRNA and positively correlated with GDF-9 ( P<0.001), Caspase-3 mRNA was negatively correlated with GDF-9 ( P<0.001). FoxO1 mRNA and GDF-9 were positively correlated with the number of high-quality embryos, while Caspase-3 mRNA was negatively correlated with the number of high-quality embryos (all P<0.001). Multiple linear regression analysis showed that after excluding confounding factors, the levels of FoxO1 mRNA, Caspase-3 mRNA, and GDF-9 were still correlated with the number of high-quality embryos (all P<0.001). IVF-ET clinical pregnancy was taken as a dependent variable and the number of high-quality embryos, basic FSH, basic LH, estradiol, FoxO1 mRNA, Caspase-3 mRNA, and GDF-9 were included as independent variables for logistic regression analysis. The number of high-quality embryos, basic FSH, basic LH, estradiol on the day of oocytes retrieved, FoxO1 mRNA, Caspase-3 mRNA and GDF-9 were significantly correlated with clinical pregnancy (all P<0.001). The area under curve (AUC) value of FoxO1 mRNA, Caspase-3 mRNA and GDF-9 mRNA combined to predict IVF-ET clinical pregnancy was 0.891, which was larger than FoxO1 mRNA alone (AUC=0.796, P=0.012). Conclusion:FoxO1 mRNA, Caspase-3 mRNA, and GDF-9 levels are related to embryonic development and sex hormone levels. Increased expression of FoxO1 mRNA and GDF-9 and decreased expression of Caspase-3 mRNA are beneficial to clinical pregnancy. Combined detection of FoxO1, Caspase-3 and GDF-9 can evaluate IVF-ET clinical pregnancy.
